Biosynthesis of reovirus-specified polypeptides: the reovirus s1 mRNA encodes two primary translation products

Journal Article · Wed May 01 00:00:00 EDT 1985 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(85)90097-2· OSTI ID:6390027

Jacobs, B L; Samuel, C E

Reovirus serotypes 1 (Lang strain) and 3 (Dearing strain) code for a hitherto unrecognized low-molecular-weight polypeptide of Mr approximately 12,000. This polypeptide (p12) was synthesized in vitro in L-cell-free protein synthesizing systems programmed with either reovirus serotype 1 mRNA, reovirus serotype 3 mRNA, or with denatured reovirus genome double-stranded RNA, and in vivo in L-cell cultures infected with either reovirus serotype. Pulse-chase experiments in vivo, and the relative kinetics of synthesis of p12 in vitro, indicate that it is a primary translation product. Fractionation of reovirus mRNAs by velocity sedimentation and translation of separated mRNAs in vitro suggests that p12 is coded for by the s1 mRNA, which also codes for the previously recognized sigma 1 polypeptide. Synthesis of both p12 and sigma 1 in vitro in L-cell-free protein synthesizing systems programmed with denatured reovirus genome double-stranded RNA also suggests that these two polypeptides can be coded by the same mRNA species. It is proposed that the Mr approximately 12,000 polypeptide encoded by the S1 genome segment be designated sigma 1bNS, and that the polypeptide previously designated sigma 1 be renamed sigma 1a.

• Research Organization: Univ. of California, Santa Barbara
• OSTI ID: 6390027
• Journal Information: Virology; (United States), Vol. 1
• Country of Publication: United States
• Language: English

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POLYPEPTIDES
BIOSYNTHESIS
VIRUSES
BIOCHEMISTRY
L CELLS
MESSENGER-RNA
METHIONINE
MICE
MOLECULAR WEIGHT
SEDIMENTATION
SULFUR 35
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
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CHEMISTRY
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DRUGS
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MAMMALS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PEPTIDES
PROTEINS
RADIOISOTOPES
RNA
RODENTS
SULFUR ISOTOPES
SYNTHESIS
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