Freeze-induced membrane ultrastructural alterations in rye (Secale cereale) leaves

Journal Article · Mon Mar 01 04:00:00 UTC 1993 · Plant Physiology; (United States)

OSTI ID:6389148

Webb, M S; Steponkus, P L ^[1]

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1. Cornell Univ., Ithaca, NY (United States)

Freezing injury in protoplasts isolated from leaves of nonacclimated rye (Secale cereale cv Puma) is associated with the formation of the inverted hexagonal (H[sub II]) phase. However, in protoplasts from cold-acclimated rye, injury is associated with the occurrence of localized deviations in the fracture plane, a lesion referred to as the [open quotes]fracture-jump lesion.[close quotes] To establish that these ultrastructural consequences of freezing are not unique to protoplasts, the authors have examined the manifestations of freezing injury in leaves of nonacclimated and cold-acclimated rye by freeze-fracture electron microscopy. At [minus]10[degrees]C, injury in nonacclimated leaves was manifested by the appearance of aparticulate domains in the plasma membrane, aparticulate lamellae subtending the plasma membrane, and by the frequent occurrence of the H[sub II] phase. The H[sub II] phase was not observed in leaves of cold-acclimated rye frozen to [minus]35[degrees]C. Rather, injury was associated with the occurrence of the fracture-jump lesion between the plasma membrane and closely appressed cytoplasmic membranes. Studies of the time dependence of H[sub II] phase formation in nonacclimated leaves indicated that freeze-induced dehydration requires longer times in leaves than in isolated protoplasts. These results demonstrate that the freeze-induced formation of the H[sub II] phase in nonacclimated rye and the fracture-jump lesion in cold-acclimated rye are not unique to protoplasts but also occur in the leaves from which the protoplasts are isolated. 11 refs., 6 figs.

• DOE Contract Number: FG02-84ER13214
• OSTI ID: 6389148
• Journal Information: Plant Physiology; (United States), Journal Name: Plant Physiology; (United States) Vol. 101:3; ISSN 0032-0889; ISSN PLPHAY
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL ADAPTATION
CEREALS
DEHYDRATION
ELECTRON MICROSCOPY
FREEZING
GRAMINEAE
LAMELLAE
LEAVES
LILIOPSIDA
MAGNOLIOPHYTA
MEMBRANES
MICROSCOPY
MORPHOLOGICAL CHANGES
PLANT CELLS
PLANTS
PLASMA
RYE
ULTRASTRUCTURAL CHANGES