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Processing of virus-specific glycoproteins of varicella zoster virus

Journal Article · · Virology; (United States)
; ; ; ;

Monoclonal antibodies to varicella zoster virus (VZV) glycoproteins were used to study the processing of three glycoproteins with molecular weights of 83K-94K (gp 2), 64K (gp 3), and 55K (gp 5). Immunoprecipitation experiments performed with VZV-infected cells, pulse labeled with (/sup 3/H)glucosamine in the presence of tunicamycin, suggest that O-linked oligosaccharide is present on the glycoprotein of gp 2. Use of the enzyme endo-beta-N-acetylglucosaminidase H revealed that the fully processed form of gp 3 had high-mannose type and that of gp 5 had only complex type of N-linked oligosaccharides. Experiments with monensin suggest that the precursor form (116K) of gp 3 is cleaved during the processing from Golgi apparatus to cell surface membrane. The extension of O-linked oligosaccharide chain and the complex type of N-linked oligosaccharide chains also occurs during this processing.

Research Organization:
Osaka Univ., Japan
OSTI ID:
6387463
Journal Information:
Virology; (United States), Journal Name: Virology; (United States) Vol. 1; ISSN VIRLA
Country of Publication:
United States
Language:
English

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Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMIDASES
AMINES
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
EVEN-ODD NUCLEI
FIBROBLASTS
GLUCOPROTEINS
GLUCOSAMINE
HEXOSAMINES
HEXOSES
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MICROORGANISMS
MOLECULAR WEIGHT
MONOCLONAL ANTIBODIES
MONOSACCHARIDES
NON-PEPTIDE C-N HYDROLASES
NUCLEI
ORGANIC COMPOUNDS
PARASITES
PREGNANCY
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SACCHARIDES
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VIRUSES