Assignment of simian rotavirus SA11 temperature-sensitive mutant groups B and E to genome segments

Journal Article · Wed May 01 00:00:00 EDT 1985 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(85)90118-7· OSTI ID:6387456

Gombold, J L; Estes, M K; Ramig, R F

Recombinant (reassortant) viruses were selected from crosses between temperature-sensitive (ts) mutants of simian rotavirus SA11 and wild-type human rotavirus Wa. The double-stranded genome RNAs of the reassortants were examined by electrophoresis in Tris-glycine-buffered polyacrylamide gels and by dot hybridization with a cloned DNA probe for genome segment 2. Analysis of replacements of genome segments in the reassortants allowed construction of a map correlating genome segments providing functions interchangeable between SA11 and Wa. The reassortants revealed a functional correspondence in order of increasing electrophoretic mobility of genome segments. Analysis of the parental origin of genome segments in ts+ SA11/Wa reassortants derived from the crosses SA11 tsB(339) X Wa and SA11 tsE(1400) X Wa revealed that the group B lesion of tsB(339) was located on genome segment 3 and the group E lesion of tsE(1400) was on segment 8.

• Research Organization: Baylor College of Medicine, Houston, TX
• OSTI ID: 6387456
• Journal Information: Virology; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
SIMIAN VIRUS
GENETIC MAPPING
MUTANTS
GENOTYPE
PHOSPHORUS 32
RNA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ISOTOPES
LIGHT NUCLEI
MAPPING
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
RADIOISOTOPES
VIRUSES
550701* - Microbiology- Tracer Techniques