Anti-tumor efficacy of lymphokine-activated killer cells and recombinant interleukin 2 in vivo

Journal Article · Mon Jul 01 04:00:00 EDT 1985 · J. Immunol.; (United States)

OSTI ID:6378873

Mule, J J; Shu, S; Rosenberg, S A

The authors showed previously that adoptive immunotherapy with the combination of LAK cells and recombinant IL 2 (RIL 2) can markedly reduce pulmonary micrometastases from multiple sarcomas established 3 days after the i.v. injection of syngeneic tumor cells in C57BL/6 mice. In this report, they analyzed the factors required for successful therapy. Titration analysis in vivo revealed an inverse relationship between the number of pulmonary metastases remaining after treatment and both the number of LAK cells and the amount of RIL 2 administered. Fresh or unstimulated splenocytes had no anti-tumor effect; a 2- to 3-day incubation of splenocytes in RIL 2 was required. LAK cells generated from allogeneic DBA (H-2d) splenocytes were as effective in vivo as syngeneic, C57BL/6 (H-2b) LAK cells. The anti-metastatic capacity of LAK cells was significantly reduced or eliminated when irradiated with 3000 rad before adoptive transfer. The combined therapy of LAK cells plus RIL 2 was shown to be highly effective in mice immunosuppressed by 500 rad total body irradiation and in treating macrometastases established in the lung 10 days after the i.v. injection of sarcoma cells. Further, reduction of both micrometastases and macrometastases could also be achieved by RIL 2 alone when administered at higher levels than were required with LAK cells. The value of LAK cell transfer and of IL 2 administration for the treatment of tumors established at other sites is currently under investigation.

• OSTI ID: 6378873
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 1; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
DOSES
EXPERIMENTAL NEOPLASMS
EXTERNAL IRRADIATION
IMMUNOSUPPRESSION
IN VIVO
IRRADIATION
KINETICS
LEUKOCYTES
LUNGS
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
METASTASES
MICE
ORGANS
RADIATION DOSES
RADIATION EFFECTS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
SPLEEN CELLS
SYNERGISM
VERTEBRATES
WHOLE-BODY IRRADIATION