Time-resolved fluorescence decay measurements for flowing particles
Patent
·
OSTI ID:6378293
Time-resolved fluorescence decay measurements are disclosed for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated CW laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes. 12 figs.
- Sponsoring Organization:
- DOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-7405-ENG-36
- Assignee:
- Univ. of California, Los Alamos, NM (United States)
- Patent Number(s):
- US 5909278; A
- Application Number:
- PPN: US 8-902395
- OSTI ID:
- 6378293
- Country of Publication:
- United States
- Language:
- English
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