Time-resolved fluorescence decay measurements for flowing particles
Patent
·
OSTI ID:6378293
Time-resolved fluorescence decay measurements are disclosed for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated CW laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes. 12 figs.
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-7405-ENG-36
- Assignee:
- Univ. of California, Los Alamos, NM (United States)
- Patent Number(s):
- US 5909278; A
- Application Number:
- PPN: US 8-902395
- OSTI ID:
- 6378293
- Resource Relation:
- Patent File Date: 29 Jul 1997
- Country of Publication:
- United States
- Language:
- English
Similar Records
Time-resolved fluorescence decay measurements for flowing particles
Fluorescence intensity and lifetime measurement of free and particle-bound fluorophore in a sample stream by phase-sensitive flow cytometry
Flow cytometer for resolving signals from heterogeneous fluorescence emissions and quantifying lifetime in fluorochrome-labeled cells/particles by phase-sensitive detection
Patent
·
Fri Jan 01 00:00:00 EST 1999
·
OSTI ID:6378293
Fluorescence intensity and lifetime measurement of free and particle-bound fluorophore in a sample stream by phase-sensitive flow cytometry
Journal Article
·
Wed Dec 01 00:00:00 EST 1999
· Review of Scientific Instruments
·
OSTI ID:6378293
Flow cytometer for resolving signals from heterogeneous fluorescence emissions and quantifying lifetime in fluorochrome-labeled cells/particles by phase-sensitive detection
Journal Article
·
Wed Dec 01 00:00:00 EST 1993
· Review of Scientific Instruments; (United States)
·
OSTI ID:6378293