Resonance Raman spectra of 13-demethylretinal bacteriorhodopsin and of a picosecond bathochromic photocycle intermediate

Journal Article · Thu Jun 14 04:00:00 EDT 1990 · Journal of Physical Chemistry; (USA)

DOI:https://doi.org/10.1021/j100375a030· OSTI ID:6378182

Noguchi, T; Kolaczkowski, S; Atkinson, G H ^[1]; Gaertner, W ^[2]

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1. Univ. of Arizona, Tucson (USA)
2. Univ. of Freiburg (West Germany)

Resonance Raman spectra of an artificial bacteriorhodopsin pigment containing a 13-demethylretinal chromophore and of a bathochromic photocycle intermediate formed within 150 ps are presented. The frequency shifts observed in spectra recorded from samples in H{sub 2}O and D{sub 2}O solvents yield vibrational assignments which identify the 13-demethyl pigment as containing a C{sub 13}{double bond}C{sub 14}cis, C{sub 15}{double bond}N syn configuration of retinal and a bathochromic intermediate at 150 ps as containing the C{sub 13}{double bond}C{sub 14} trans, C{sub 15}{double bond}N syn configuration of retinal. The relationships of the retinal structures in the 13-demethyl pigment and its 150-ps intermediate to those in BR-548, BR-570, and their respective bathochromic intermediates in the native photocycle are discussed.

• OSTI ID: 6378182
• Journal Information: Journal of Physical Chemistry; (USA), Journal Name: Journal of Physical Chemistry; (USA) Vol. 94:12; ISSN 0022-3654; ISSN JPCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400500* -- Photochemistry
CHEMISTRY
DATA
DATA ANALYSIS
EXPERIMENTAL DATA
INFORMATION
MEASURING INSTRUMENTS
MEASURING METHODS
NUMERICAL DATA
PHOTOCHEMISTRY
PHOTOSYNTHETIC BACTERIA
PIGMENTS
RAMAN SPECTRA
REACTION INTERMEDIATES
SPECTRA