Resonance Raman spectra of 13-demethylretinal bacteriorhodopsin and of a picosecond bathochromic photocycle intermediate
- Univ. of Arizona, Tucson (USA)
- Univ. of Freiburg (West Germany)
Resonance Raman spectra of an artificial bacteriorhodopsin pigment containing a 13-demethylretinal chromophore and of a bathochromic photocycle intermediate formed within 150 ps are presented. The frequency shifts observed in spectra recorded from samples in H{sub 2}O and D{sub 2}O solvents yield vibrational assignments which identify the 13-demethyl pigment as containing a C{sub 13}{double bond}C{sub 14}cis, C{sub 15}{double bond}N syn configuration of retinal and a bathochromic intermediate at 150 ps as containing the C{sub 13}{double bond}C{sub 14} trans, C{sub 15}{double bond}N syn configuration of retinal. The relationships of the retinal structures in the 13-demethyl pigment and its 150-ps intermediate to those in BR-548, BR-570, and their respective bathochromic intermediates in the native photocycle are discussed.
- OSTI ID:
- 6378182
- Journal Information:
- Journal of Physical Chemistry; (USA), Journal Name: Journal of Physical Chemistry; (USA) Vol. 94:12; ISSN 0022-3654; ISSN JPCHA
- Country of Publication:
- United States
- Language:
- English
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