Technetium-99m labeling of antibodies to cardiac myosin Fab and to human fibrinogen

Journal Article · Mon Nov 01 04:00:00 EST 1982 · J. Nucl. Med.; (United States)

OSTI ID:6377182

Khaw, B A; Strauss, H W; Carvalho, A; Locke, E; Gold, H K; Haber, E

A method of labeling biologically active labile macromolecules, such as human fibrinogen (HF) and anticardiac-myosin Fab (AM-Fab), with Tc-99m at neutral pH was developed. This method uses dithionite reduction of pertechnetate and subsequent labeling to test the method with acid-labile macromolecules. Complexes of diethylene triamine pentaacetic acid with macromolecules such as human fibrinogen (D-HF) and anticardiac-myosin Fab (D-AM-Fab) were labeled and utilized in in vitro and in vivo studies. In biodistribution studies, the Tc-99m D-HF had a two-component blood clearance (half-times 1 hr and 15 hr) and was 80-88% coagulable. The Tc-99m AM-Fab retained its immunoreactivity as tested by affinity chromatography; also during in vivo localization in experimental myocardial infarction. This labeling technique provides an easy and efficient approach to the Tc-99m labeling of other biologically active and acid-labile macromolecules.

• Research Organization: Massachusetts General Hospital, Boston
• OSTI ID: 6377182
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 23:11; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BLOOD COAGULATION FACTORS
BLOOD-PLASMA CLEARANCE
CARDIOVASCULAR DISEASES
CHEMICAL PREPARATION
CLEARANCE
COAGULANTS
DISEASES
DRUGS
FIBRINOGEN
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
HOURS LIVING RADIOISOTOPES
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MYOCARDIAL INFARCTION
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PERTECHNETATES
PROTEINS
RADIOISOTOPES
RADIOPHARMACEUTICALS
SYNTHESIS
TECHNETIUM 99
TECHNETIUM COMPOUNDS
TECHNETIUM ISOTOPES
TRANSITION ELEMENT COMPOUNDS
YEARS LIVING RADIOISOTOPES