Insulin, concanavalin A, EGF, IFG-I and vanadate activate de novo phosphatidic acid and diacylglycerol synthesis, C-kinase, and glucose transport in BC3H-1 myocytes

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6374349

Cooper, D R; Hernandez, H; Konda, T S; Standaert, M S; Pollet, R J; Farese, R V

The authors have reported that insulin stimulates de novo synthesis of phosphatidic acid (PA) which is metabolized directly to diacylglycerol (DG) in BS3H-1 myocytes; this is accompanied by increases in C-kinase activity in membrane and cytosolic extracts. This pathway may be involved in stimulating glucose transport and other metabolic processes. In this study, the authors have compared the effects of concanavalin A, EGF, IGF-I and sodium orthovanadate to insulin on PA/DG synthesis, C-kinase activity and glucose transport. All were found to be effective in stimulating glucose transport. Additionally, all activators rapidly increased the incorporation of (/sup 3/H)glycerol into DG and total glycerolipids, although none were as effective as insulin, which increased (/sup 3/H)DG 400% in 1 minute. Increased incorporation into phospholipids and triacylglycerols and to a lesser extent monoacylglycerol was also noted. They examined effects of concanavalin A and EGF on C-kinase activity and found that both agonists, like insulin, increase C-kinase activity in cytosolic and/or membrane fractions. Their findings raise the possibility that activation of receptors having associated tyrosine kinase activity may provoke some cellular responses through de novo PA/GD synthesis and C-kinase activation.

• Research Organization: Univ. of South Florida School of Medicine, Tampa
• OSTI ID: 6374349
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ALCOHOLS
ALDEHYDES
ANTIBODIES
BIOLOGICAL FUNCTIONS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBOHYDRATES
COMPARATIVE EVALUATIONS
CONCANAVALIN
ELEMENTS
ENZYME ACTIVITY
ENZYMES
FUNCTIONS
GLUCOSE
GLYCEROL
GROWTH FACTORS
HEMAGGLUTININS
HEXOSES
HORMONES
HYDROXY COMPOUNDS
INSULIN
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LECTINS
MEMBRANE TRANSPORT
METALS
MITOGENS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
SACCHARIDES
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
TRANSITION ELEMENTS
TRITIUM COMPOUNDS
VANADIUM