Stable expression and replication of hepatitis B virus genome in an integrated state in a human hepatoma cell line transfected with the cloned viral DNA
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
A human hepatocellular carcinoma cell line (Huh6-c15) was transfected with a recombinant DNA molecule that consists of tandemly arranged hepatitis B virus (HBV) genome and a neomycin-resistant gene. One clone resistant to G-418 produces and releases surface antigen and e antigen into medium at a high level and accumulates core particles intracellularly. This clone has a chromosomally integrated set of the original recombinant DNA and produces a 3.5-kilobase transcript corresponding to the pregenome RNA as well as HBV DNAs in an extrachromosomal form. Most of these DNAs were in single-stranded or partially double-stranded form and were packaged in the intracellular core particles. In the medium, particles were detected that contained HBV DNA and were morphologically indistinguishable from Dane particles. These results demonstrate that the HBV genome in an integrated state acted as a template for viral gene expression and replication. The cells were maintained for more than 6 months without losing the ability to produce the extrachromosomal HBV DNA and Dane-like particles. Thus, the cells can be used as a model system for analyses of gene expression and DNA replication of HBV in human hepatocytes.
- Research Organization:
- Osaka Univ., Japan
- OSTI ID:
- 6372622
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:2; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Overexpression of human ACE2 protein in mouse fibroblasts stably transfected with the intact ACE2 gene
Transformation of human epidermal cells by transfection with plasmid containing simian virus 40 DNA linked to a neomycin gene is a defined medium
Novel stable HBV producing cell line systems for expression and screening antiviral inhibitor of hepatitis B virus in human hepatoma cell line
Journal Article
·
Tue Jan 30 19:00:00 EST 2024
· Virology
·
OSTI ID:2338131
Transformation of human epidermal cells by transfection with plasmid containing simian virus 40 DNA linked to a neomycin gene is a defined medium
Journal Article
·
Sat Dec 31 23:00:00 EST 1988
· Experimental Cell Research; (United States)
·
OSTI ID:5745299
Novel stable HBV producing cell line systems for expression and screening antiviral inhibitor of hepatitis B virus in human hepatoma cell line
Journal Article
·
Thu Mar 15 00:00:00 EDT 2018
· Biochemical and Biophysical Research Communications
·
OSTI ID:23137268
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM DISEASES
DISEASES
DNA REPLICATION
ELECTRON MICROSCOPY
ELECTROPHORESIS
GENE REGULATION
HEPATITIS
HEPATOMAS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
LIVER CELLS
MESSENGER-RNA
MICROORGANISMS
MICROSCOPY
NEOPLASMS
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
RADIOISOTOPES
RNA
SOMATIC CELLS
TRANSCRIPTION
TRANSMISSION ELECTRON MICROSCOPY
TUMOR CELLS
VIRUSES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM DISEASES
DISEASES
DNA REPLICATION
ELECTRON MICROSCOPY
ELECTROPHORESIS
GENE REGULATION
HEPATITIS
HEPATOMAS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
LIVER CELLS
MESSENGER-RNA
MICROORGANISMS
MICROSCOPY
NEOPLASMS
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
RADIOISOTOPES
RNA
SOMATIC CELLS
TRANSCRIPTION
TRANSMISSION ELECTRON MICROSCOPY
TUMOR CELLS
VIRUSES