Transition-metal compounds as new reagents for selective cross-linking of proteins. Synthesis and characterization of two bis(cytochrome c) complexes of platinum
This study shows, for the first time, that proteins can be cross-linked selectively via transition-metal compounds to form stable protein complexes. Incubation of horse cytochrome c (designated cyt) with reagents PtCl4S with trans-(Pt(2-Fpy)2Cl2) under mild conditions yields stable diprotein complexes trans-(PtCl2(cyt)2) and trans-(Pt(2-Fpy)2(cyt)2), respectively (2-Fpy is 2-fluoropyridine). The complexes are purified and characterized chromatographically. The protein molecules are coordinated to the Pt(II) atom through the thioether side chains of their respective Met 65 residues. Spectroscopic and electrochemical measurements indicate that, except for the slightly increased abundance of the high-spin form of ferriheme, the structural and redox properties of the cytochrome c molecules remain unaltered upon cross linking. The diprotein complexes are stable indefinitely under ordinary conditions and yet they can be cleaved, and the native protein restored, in a mild reaction. Platinum compounds hold promise as selective and versatile reagents for cross-linking of proteins. 54 references, 1 figure, 3 tables.
- Research Organization:
- Iowa State Univ., Ames
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 6371926
- Journal Information:
- Inorg. Chem.; (United States), Journal Name: Inorg. Chem.; (United States) Vol. 26:13; ISSN INOCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400201* -- Chemical & Physicochemical Properties
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CATALYTIC EFFECTS
CHEMICAL PREPARATION
CHEMICAL REACTIONS
COMPLEXES
CROSS-LINKING
CYTOCHROMES
DATA
EXPERIMENTAL DATA
INFORMATION
NUMERICAL DATA
ORGANIC COMPOUNDS
PIGMENTS
PLATINUM COMPLEXES
POLYMERIZATION
PROTEINS
SYNTHESIS
TRANSITION ELEMENT COMPLEXES