Effect of dose fractionation on the ethylnitrosourea induction of specific-locus mutations in mouse spermatogonia

Journal Article · Tue Jun 01 00:00:00 EDT 1982 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.79.11.3592· OSTI ID:6369128

Russell, W L; Hunsicker, P R; Carpenter, D A; Cornett, C V; Guinn, G M

As measured by specific-locus mutations in mouse spermatogoniz, fractionating a dose of 100 mg of ethylnitrosourea per kg of body weight into doses of 10 mg/kg injected intraperitoneally at weekly intervals greatly reduces the mutation frequency compared with that from a single dose of 100 mg/kg. Because there is independent evidence that the doses of 10 and 100 mg/kg reach the germ cells in amounts directly proportional to the injected dose, the lower mutational response with the fractionated dose is attributed to repair. The induced mutation rate expected from a single 10-mg/kg dose (on the assumption that this would be 1/10th the rate observed after 10 such doses) would be only 75% of the spontaneous mutation rate. Mouse spermatogonia apparently have an efficient repair system that is effective even against a potent mutagen.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6369128
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 79
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
NITROSO COMPOUNDS
BIOLOGICAL EFFECTS
ACUTE EXPOSURE
CHRONIC EXPOSURE
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
GENE MUTATIONS
MICE
MUTATION FREQUENCY
SPERMATOGONIA
ANIMALS
DATA
GAMETES
GERM CELLS
INFORMATION
MAMMALS
MUTATIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RODENTS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550400 - Genetics