Deletion analysis of the expression of rRNA genes and associated tRNA genes carried by a lambda transducing bacteriophage. [uv radiation, Escherichia coli]
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:6367302
Transducing phage lambda ilv5 carries genes for rRNA's, spacer tRNA's (tRNA/sub 1//sup Ile/ and tRNA/sub 1B//sup Ala/), and two other tRNA's (tRNA/sub 1//sup Asp/ and tRNA/sup Trp/). We have isolated a mutant of lambda ilv5, lambda ilv5su7, which carries an amber suppressor mutation in the tRNA/sup Trp/ gene. A series of deletion mutants were isolated from the lambda ilv5su7 phage. Genetic and biochemical analyses of these deletion mutants have confirmed our previous conclusion that the genes for tRNA/sub 1//sup Asp/ and tRNA/sup Trp/ located at the distal end of the rRNA operon (rrnC) are cotranscribed with other rRNA genes in that operon. In addition, these deletions were used to define roughly the physical location of the promoter(s) of the rRNA operon carried by the lambda ilv5su7 transducing phage.
- Research Organization:
- Univ. of Wisconsin, Madison
- OSTI ID:
- 6367302
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 137:1; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400 -- Genetics
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGY
BIOSYNTHESIS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENES
GENETICS
MICROORGANISMS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
RNA
SYNTHESIS
ULTRAVIOLET RADIATION
VIRUSES
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGY
BIOSYNTHESIS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENES
GENETICS
MICROORGANISMS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
RNA
SYNTHESIS
ULTRAVIOLET RADIATION
VIRUSES