Correlation between production of benzo(A)pyrene metabolites and BPDE I-DG adduct levels in human epithelial cells in vitro pretreated with cytochrome P450 inhibitors or inducer

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6365861

Lehman, T A; Milo, G E

Human epidermal keratinocytes were established from neonatal foreskins. Cultures were pretreated for 24 hr with either butylated hydroxyanisole (BHA), methyl butylated hydroxyanisole (MeBHA) or 7,8 benzoflavone (7,8BF). For metabolite detection studies, cultures were treated with radiolabeled benzo(a)pyrene (BP) for 24 hr. Ethyl acetate soluble metabolites were extracted for HPLC analysis. BHA and 7,8BF pretreatment both significantly decreased intracellular production of 7,8 diol BP compared to cultures treated only with radiolabeled BP. MeBHA pretreatment greatly increased intracellular 7,8 diol BP formation compared to BP treated controls. For DNA adduct analysis, cultures were pretreated as described above, and then treated for 24 hr with non-radiolabeled BP. Cellular DNA was isolated and /sup 32/P-postlabeled for adduct analysis. Cultures pretreated with either BHA or 7,8BF formed significantly fewer BPDE I-dG adducts than nonpretreated cultures, while cultures pretreated with MeBHa formed more BPDE I-dG adducts. Thus, BHA and 7,8BF act similarly in reducing BP activation and adduct formation while MeBHa, a structural analog of BHA, increases BP activation and adduct formation in human keratinocyte cultures in vitro.

• Research Organization: Ohio State Univ., Columbus
• OSTI ID: 6365861
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANISOLE
AROMATICS
BENZOPYRENE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CHROMATOGRAPHY
CONDENSED AROMATICS
DAYS LIVING RADIOISOTOPES
DNA
DNA ADDUCTS
ENZYME INHIBITORS
EPITHELIUM
ETHERS
FLAVENOIDS
FLAVONES
GENETIC EFFECTS
HYDROCARBONS
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
METABOLISM
METABOLITES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
SEPARATION PROCESSES
TISSUES
TRACER TECHNIQUES
VERTEBRATES