Functional reconstitution of receptors for bradykinin and des argZ-bradykinin from pulmonary artery membranes

Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6360258

Cahill, M C; Polgar, P; Dickey, B F; Fishman, J B

Bradykinin (BK) is a vasoactive peptide which mediates a number of vascular functions, including activation of prostaglandin biosynthesis and modulation of vasomotor tone. BK and its kinase II metabolite, des argZ-BK, have been reported to activate the B2 and B1 receptors, respectively. The authors prepared membranes from the bovine pulmonary artery and solubilized membrane proteins using the zwitterionic detergent CHAPS (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate). The solubilized proteins were reconstituted into liposomes via a gel filtration method. The vesicles specifically bound both TH-BK and TH-des argZ-BK, although the latter bound with significantly lower affinity. The binding of TH-BK was inhibited 65% by guanosine 5'-0-thiotriphosphate S while the binding of TH-des argZ-BK was unaffected. This suggests that the receptor for BK was associated with a guanine-nucleotide binding protein whereas the receptor for des argZ-BK was not. Since des argZ-BK has recently been reported to be considerably less potent than BK at activating the turnover of phosphatidylinositol, the authors data suggest that this is due to the des argZ-BK receptor not being coupled to a G-protein. Further work towards characterizing these receptors is now underway.

• Research Organization: Boston Univ. Medical Center, MA
• OSTI ID: 6360258
• Report Number(s): CONF-870644-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 46:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
ARTERIES
BIOCHEMICAL REACTION KINETICS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CATTLE
CELL CONSTITUENTS
CELL MEMBRANES
DOMESTIC ANIMALS
GUANYLIC ACID
ISOTOPE APPLICATIONS
KINETICS
KININS
LABELLED COMPOUNDS
LUNGS
MAMMALS
MEMBRANES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
REACTION KINETICS
RECEPTORS
RESPIRATORY SYSTEM
RUMINANTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES