Identification and characterization of luekotriene C/sub 4/ and D/sub 4/ receptors on a cultured smooth muscle cell line, BC3H-1
The authors studied the characteristics of the leukotriene (LT) C/sub 4/ and D/sub 4/ receptors on a cultured smooth muscle cell line, BC3H-1. Specific (/sup 3/H)LTC/sub 4/ binding to the cell membrane was greater than 80% of total binding and saturable at a density of 3.96 +/- 0.39 pmol/mg protein, with an apparent dissociation constant(Kd) of 14.3 +/- 2.0 nM (n=9). The association and dissociation of (/sup 3/H)LTC/sub 4/ binding were rapid and apparent equilibrium conditions were established within 5 min. Calculated Kd value of (/sup 3/H)LTC binding from the kinetic analysis was 9.9 nM. From the competition analysis, calculated Ki value of unlabeled LTC/sub 4/ to compete for the specific binding of (/sup 3/H)LTC/sub 4/ was 9.2 nM and was in good agreement with the Kd value obtained from the Scatchard plots or kinetic analysis. The maximum number of binding sites (Bmax) of (/sup 3/H)LTD/sub 4/ in the membrane of BC3H-1 cell line was about 11 times lower than that of the (/sup 3/H)LTC/sub 4/. The calculated values of Kd and Bmax of (/sup 3/H)LTD/sub 4/ binding were 9.3 +/- 0.8 nM and 0.37 +/- 0.04 pmol/mg proteins, respectively (n=3). These findings demonstrate that BC3H-1 cell line possess both LTC/sub 4/ and LTD/sub 4/ receptors with a predominance of LTC/sub 4/ receptors. Thus, BC3H-1 cell line is a good model to study the regulation of LTC/sub 4/ and LTD/sub 4/ receptors. 34 references, 5 figures, 1 table.
- Research Organization:
- Creighton Univ. School of Medicine, Omaha, NE
- OSTI ID:
- 6355577
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 41:2; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Characterization of guinea pig myocardial leukotriene C4 binding sites. Regulation by cations and sulfhydryl-directed reagents
Binding of leukotriene C/sub 4/ to rat lung fibroblasts and stimulation of collagen synthesis in vitro
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
COMPARATIVE EVALUATIONS
DATA
EXPERIMENTAL DATA
FUNCTIONS
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MEMBRANE PROTEINS
MEMBRANES
MONOCARBOXYLIC ACIDS
MUSCLES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
RECEPTORS
TRACER TECHNIQUES
TRITIUM COMPOUNDS