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Enzymatic activities in ultraviolet-induced DNA degradation in Escherichia coli. [Genetic variations in activity of exonucleases and polymerases in E coli mutants]

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3575145· OSTI ID:6355190
;

Ultraviolet-induced DNA degradation is found to occur in Escherichia coli WP6 polA1 and K-12 RS polA exl strains with the former strain degrading its DNA more extensively after 20 J/m/sup 2/. K-12 SR78 recB21 degrades very little or no DNA after 20 J/m/sup 2/. At a higher fluence of 60 J/m/sup 2/, however, about 20% of the DNA is degraded, albeit at a lower rate compared to the wild-type DY998. These data suggest that uvrA-dependent degradation involves the 5' ..-->.. 3' exonuclease activity of polymerase I (exonuclease VI) and recBC exonuclease V activity, with the latter less crucial after exposure to a larger fluence. In WP67 uvrA polA1 strains, uv-induced DNA degradation after 10 J/m/sup 2/ is abolished unless the cells are incubated in the presence of chloramphenicol. In K-12 DM20 uvrA recB21 strains, post-uv incubation after 10 J/m/sup 2/ with or without chloramphenicol does not result in DNA degradation, suggesting that chloramphenicol-enhancible degradation involves primarily recBC exonuclease activity.

Research Organization:
Indiana Univ., Indianapolis
OSTI ID:
6355190
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 77:2; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGICAL VARIABILITY
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
DATA
DATA FORMS
DECOMPOSITION
DNA
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
EXPERIMENTAL DATA
GENETIC VARIABILITY
INFORMATION
IRRADIATION
ISOLATED VALUES
KINETICS
MICROORGANISMS
MUTANTS
NUCLEASES
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PHOSPHOTRANSFERASES
POLYMERASES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RADIOLYSIS
REACTION KINETICS
RECOVERY
REPAIR
STRAND BREAKS
TRANSFERASES
ULTRAVIOLET RADIATION