Identification of bromodeoxyuridine in malignant and normal cells following therapy: relationship to complications

Journal Article · Wed Aug 01 04:00:00 EDT 1984 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(84)90365-1· OSTI ID:6354606

Morstyn, G; Kinsella, T; Hsu, S M; Russo, A; Gratzner, H; Mitchell, J

During a clinical Phase I study of bromodeoxyuridine (BUdR) as a radiation sensitizer the authors identified the normal and malignant cells that incorporated the BUdR. BUdR was infused for up to 14 days and the in vivo incorporation of BUdR into DNA was assessed using an immonohistochemical technique and a monoclonal antibody directed against BUdR. BUdR was identified in 50% of breast cancer cells and 10% of cells in a malignant melanoma. BUdR was also found in the basal layer of the normal epidermis and in 50% of cells in the marrow. The incorporation of BUdR into cells in the epidermis and marrow may produce the phototoxicity and myelosuppression observed in patients treated with BUdR. Sequential biopsies from a breast cancer taken prior to and following radiotherapy suggested that cells that incorporated BUdR may have been selectively killed by the radiation. The immunohistochemical technique used to identify BUdR appeared to be useful for studies of in vivo and in vitro cell proliferation.

• Research Organization: Univ. of Miami Hospitals and Clinics, FL
• OSTI ID: 6354606
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 10:8; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

550603 -- Medicine-- External Radiation in Therapy-- (1980-)
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANTIBODIES
ANTIMETABOLITES
AZINES
BIOLOGICAL ACCUMULATION
BODY
BONE MARROW CELLS
BROMOURACILS
BUDR
CONNECTIVE TISSUE CELLS
CYTOLOGICAL TECHNIQUES
DNA
DRUGS
EPIDERMIS
EPITHELIUM
FRACTIONATED IRRADIATION
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOSUPPRESSION
IRRADIATION
MEDICINE
MONOCLONAL ANTIBODIES
NUCLEAR MEDICINE
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIOLOGY
RADIOSENSITIVITY EFFECTS
RADIOTHERAPY
RIBOSIDES
SIDE EFFECTS
SKIN
SOMATIC CELLS
THERAPY
TISSUES
TUMOR CELLS
URACILS