Human HSP70 promoter contains at least two distinct regulatory domains
The expression of the human HSP70 gene is induced by a wide range of physiological stresses, including exposure to heat shock and heavy metals, or under nonstress conditions, such as in response to serum stimulation. The authors have previously demonstrated that in either case the regulated expression is at the primary level of transcription. To determine whether transcription is mediated through a single or multiple genetic elements, they have dissected the sequences upstream of the transcription start site of the human HSP70 gene by constructing chimeric genes retaining variable amounts of 5' flanking regions fused to the bacterial gene encoding chloramphenicol acetyltransferase. Transcription from the chimeric genes was determined by S1 nuclease analysis of separate stable transfectants. The sequences required for heat shock and cadmium induction lie between -107 and -68. Within this region is the sequence CTGGAATATTCCCG, which is identical in 12/14 positions with the heat shock element of Drosophila heat shock genes, and a separate sequence, CGNCCCGG, which is homologous to the core of the human metallothionein II metal-responsive element. The sequences required for serum-stimulated transcription are distinct from the heat shock element. The human HSP70 promoter contains at least two regulatory domains - a distal domain responsive to heat shock or cadmium and a proximal domain responsive to stimulation by serum.
- Research Organization:
- Northwestern Univ., Evanston, IL (United States)
- OSTI ID:
- 6352876
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 83:3
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CADMIUM SULFATES
GENETIC EFFECTS
GENE REPRESSORS
DNA SEQUENCING
HEAT
GENE REGULATION
PLASMIDS
TRANSCRIPTION
TRANSFERASES
BIOLOGICAL EFFECTS
CADMIUM COMPOUNDS
CELL CONSTITUENTS
ENERGY
ENZYMES
NUCLEOPROTEINS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
SULFATES
SULFUR COMPOUNDS
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560300 - Chemicals Metabolism & Toxicology
560200 - Thermal Effects