Aerobic oxidation of hydrogen sulfide by Thiobacillus denitrificans

Journal Article · Wed Apr 01 04:00:00 EST 1987 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260290605· OSTI ID:6351100

Sublette, K L

It has been demonstrated that Thiobacillus denitrificans may be readily cultured aerobically in batch and continuous flow reactors on H/sub 2/S(g) under sulfide limiting conditions. Under these conditions sulfide concentrations in the culture medium were less than 1..mu..M resulting in very low concentrations of H/sub 2/S in the reactor outlet gas. Biomass yield under aerobic conditions was much lower than previously reported for anaerobic conditions, presumably because of oxygen inhibition of growth. However, biomass yield was not affected by steady state oxygen concentration in the range of 45..mu..M-150..mu..M. Biomass yield was also observed to be essentially independent of specific growth rate in the range of 0.030-0.053 h/sup -1/. Indicators of reactor upset were determined and recovery from upset conditions demonstrated. Maximum loading of the biomass from H/sub 2/S oxidation under aerobic conditions was observed to be 15.1-20.9 mmol/h/g biomass which is much higher than previously reported for anaerobic conditions. Other aspects of the stoichiometry of aerobic H/sub 2/S oxidation are also reported.

• Research Organization: Lummus Crest, Inc., Bloomfield, NJ
• OSTI ID: 6351100
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 29:6; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROBIC CONDITIONS
BACTERIA
CHALCOGENIDES
CHEMICAL REACTIONS
GROWTH
HYDROGEN COMPOUNDS
HYDROGEN SULFIDES
MICROORGANISMS
OXIDATION
STOICHIOMETRY
SULFIDES
SULFUR COMPOUNDS
SULFUR-OXIDIZING BACTERIA