Tc-99m galactosyl-neoglycoalbumin: in vitro characterization of receptor-mediated binding

Journal Article · Sun Jul 01 00:00:00 EDT 1984 · J. Nucl. Med.; (United States)

OSTI ID:6348671

Vera, D R; Krohn, K A; Stadalnik, R C; Scheibe, P O

Hepatic binding protein (HBP) is a membrane receptor that binds and transports plasma glycoproteins from hepatic blood to hepatocellular lysosomes. A characterization is made of the in vitro binding of Tc-99m galactosyl-neoglycoalbumin (Tc-NGA), a synthetic HBP ligand, to liver membrane. Structural modifications of NGA resulted in the alteration of the equilibrium constant, KA, and the forward-binding rate constant, kb. Binding was second-order; the relative amount of membrane-bound NGA depended on the initial concentrations of ligand and membrane. Membrane displacement studies, using carrier ligands in contrast to previously bound Tc-NGA or I-NGA, correlated with the binding characteristics of a native HBP ligand, asialo-orosomucoid. Computer simulation was used to study the detectability of the changes in HBP concentration at different values of kb. The simulations indicated that radiopharmacokinetic sensitivity to alterations in (HBP) should be possible using a neoglycoalbumin preparation with a carbohydrate density within the range of 15 to 25 galactose units per albumin molecule.

• Research Organization: Univ. of California, Davis
• OSTI ID: 6348671
• Journal Information: J. Nucl. Med.; (United States), Vol. 25:7
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOPROTEINS
BIOCHEMICAL REACTION KINETICS
RECEPTORS
BIOLOGICAL MODELS
BLOOD
CELL MEMBRANES
COMPUTERIZED SIMULATION
ISOMERIC NUCLEI
LABELLED COMPOUNDS
LIGANDS
LIVER
TECHNETIUM 99
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBOHYDRATES
CELL CONSTITUENTS
DIGESTIVE SYSTEM
GLANDS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MATERIALS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
SIMULATION
TECHNETIUM ISOTOPES
YEARS LIVING RADIOISOTOPES
551001* - Physiological Systems- Tracer Techniques