Induction of L-phenylalanine ammonia-lyase during utilization of phenylalanine as a carbon or nitrogen source in Rhodotorula glutinis
Rhodotorula glutinis is a convenient source of L-phenylalanine ammonia-lyase, an enzyme that is useful as a biochemical reagent in the assay of L-phenylalanine. There have been previous descriptions of induced lyase production in complex medium where induction occurs late in exponential growth, suggesting a role in secondary metabolism such as is the case in higher plants. A higher specific activity of L-phenylalanine ammonia-lyase (sixfold higher than in complex medium) can be obtained during midexponential growth in a defined medium containing L-phenylalanine as the sole source of carbon. L-phenylalanine will also induce lyase synthesis during exponential growth in minimal medium in which L-phenylalanine is the sole source of nitrogen. The appearance of lyase in complex medium supplemented with L-phenylalanine is probably triggered fortuitously by exhaustion late in growth of a prime source of nitrogen. In this study, R. glutinis appeared to express a single lyase enzyme, regardless of whether induction was nitrogen signaled or carbon signaled. Thin-layer chromatographic analysis of ether extracts prepared fom cultures induced with doubly labeled (U-/sup 14/C; ring-4-/sup 3/H) L-phenylalanine provided evidence of a catabolic sequence containing cinnamic acid, benzoic acid, and 4-hydroxybenzoic acid as degradative intermediates. 3,4-Dihydroxybenzoic acid was not identified as a catabolic intermediate.
- Research Organization:
- State Univ. of New York, Binghamton
- DOE Contract Number:
- EP-78-S-02-4967
- OSTI ID:
- 6338130
- Journal Information:
- J. Bacteriol.; (United States), Vol. 146:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LYASES
ENZYME ACTIVITY
PHENYLALANINE
METABOLISM
AMMONIA
BENZOIC ACID
BIOSYNTHESIS
CARBON
CARBON 14 COMPOUNDS
CELL CYCLE
CINNAMIC ACID
CULTURE MEDIA
EXPERIMENTAL DATA
NITROGEN
NUTRIENTS
REACTION INTERMEDIATES
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
YEASTS
AMINO ACIDS
CARBOXYLIC ACIDS
DATA
ELEMENTS
ENZYMES
FUNGI
HYDRIDES
HYDROGEN COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NONMETALS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques
550701 - Microbiology- Tracer Techniques