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Title: Determination of the anomeric specificity of the Escherichia coli CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase by TC NMR spectroscopy

Abstract

(99%, 1- TC)- and (90%, 2- TC)3-deoxy-D-manno-octulosonic acid (KDO) were prepared enzymatically and used to determine the anomeric specificity of the CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyl transferase (CMP-KDO synthetase) by TC NMR spectroscopy. Addition of CMP-KDO synthetase to reaction mixtures containing either 1- TC- or 2- TC-labeled KDO resulted in rapid CMP-KDO formation which was accompanied by a substantial decrease in the TC-enriched resonances of the beta-pyranose form of KDO relative to the resonances of other KDO species in solution, demonstrating that the beta-pyranose is the preferred substrate. Concomitant with the production of CMP-KDO was the appearance of peaks at 174.3 and 101.4 ppm when (1- TC)- and (2- TC)KDO, respectively, were used as substrates. The correspondence of these resonances to the enriched carbons in CMP-KDO was confirmed by the expected 3-bond (3JP,C-1 = 6.9 Hz) and 2-bond coupling (2JP,C-2 = 8.3 Hz) between the labeled carbons and the ketosidically linked phosphoryl group. A large coupling (3J = 5.7 Hz) was observed in proton-coupled spectra of CMP-(1- TC)KDO between carbon 1 and the axial proton at carbon 3 of KDO. The magnitude of this coupling constant supports a diaxial relationship between these two groups and, along with chemical shift data, indicates that KDOmore » retains the beta-configuration when linked in CMP-KDO.« less

Authors:
;
Publication Date:
Research Org.:
Abbott Labs., Abbott Park, IL
OSTI Identifier:
6336275
Resource Type:
Journal Article
Journal Name:
J. Biol. Chem.; (United States)
Additional Journal Information:
Journal Volume: 27
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; NUCLEOTIDYLTRANSFERASES; RECEPTORS; CARBON 13; ESCHERICHIA COLI; NUCLEAR MAGNETIC RESONANCE; SUBSTRATES; BACTERIA; CARBON ISOTOPES; ENZYMES; EVEN-ODD NUCLEI; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MICROORGANISMS; NUCLEI; PHOSPHORUS-GROUP TRANSFERASES; RESONANCE; STABLE ISOTOPES; TRANSFERASES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Kohlbrenner, W E, and Fesik, S W. Determination of the anomeric specificity of the Escherichia coli CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase by TC NMR spectroscopy. United States: N. p., 1985. Web.
Kohlbrenner, W E, & Fesik, S W. Determination of the anomeric specificity of the Escherichia coli CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase by TC NMR spectroscopy. United States.
Kohlbrenner, W E, and Fesik, S W. Mon . "Determination of the anomeric specificity of the Escherichia coli CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase by TC NMR spectroscopy". United States.
@article{osti_6336275,
title = {Determination of the anomeric specificity of the Escherichia coli CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase by TC NMR spectroscopy},
author = {Kohlbrenner, W E and Fesik, S W},
abstractNote = {(99%, 1- TC)- and (90%, 2- TC)3-deoxy-D-manno-octulosonic acid (KDO) were prepared enzymatically and used to determine the anomeric specificity of the CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyl transferase (CMP-KDO synthetase) by TC NMR spectroscopy. Addition of CMP-KDO synthetase to reaction mixtures containing either 1- TC- or 2- TC-labeled KDO resulted in rapid CMP-KDO formation which was accompanied by a substantial decrease in the TC-enriched resonances of the beta-pyranose form of KDO relative to the resonances of other KDO species in solution, demonstrating that the beta-pyranose is the preferred substrate. Concomitant with the production of CMP-KDO was the appearance of peaks at 174.3 and 101.4 ppm when (1- TC)- and (2- TC)KDO, respectively, were used as substrates. The correspondence of these resonances to the enriched carbons in CMP-KDO was confirmed by the expected 3-bond (3JP,C-1 = 6.9 Hz) and 2-bond coupling (2JP,C-2 = 8.3 Hz) between the labeled carbons and the ketosidically linked phosphoryl group. A large coupling (3J = 5.7 Hz) was observed in proton-coupled spectra of CMP-(1- TC)KDO between carbon 1 and the axial proton at carbon 3 of KDO. The magnitude of this coupling constant supports a diaxial relationship between these two groups and, along with chemical shift data, indicates that KDO retains the beta-configuration when linked in CMP-KDO.},
doi = {},
journal = {J. Biol. Chem.; (United States)},
number = ,
volume = 27,
place = {United States},
year = {1985},
month = {11}
}