Enzymatic hydrolysis of cellulose in aqueous two-phase systems. 1. Partition of cellulases from Trichoderma reesei

Journal Article · Mon Jul 01 04:00:00 EDT 1985 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260270715· OSTI ID:6335463

Tjerneld, F; Persson, J; Albertsson, P A; Hahn-Haegerdal, B

The partitioning of endo-..beta..-glucanase, exo-..beta..-glucananse, and ..beta..-glucosidase from Trichoderma reesei QM 9414 in aqueous two-phase systems has been studied with the object of designing a phase system for continuous bioconversion of cellulose. The partitioning of the enzymes in two-phase systems composed of various water soluble polymeric compounds were studied. Systems based on dextran and polyethylene glycol (PEG) were optimal for one sidedly partitioning of the enzymes to the bottom phase. The influence of polymer molecular weights, polymer concentration, ionic composition of the medium, pH, temperature, and adsorption of the enzymes to cellulose on the enyzme partition coefficients (K) were studied. By combining the effects of polymer molecular weight and adsorption to cellulose, K values could be reduced for endo-..beta..-glucanase to 0.02 and for ..beta..-glucosidase to 0.005 at 20 degrees C in a phase system of Dvalues could be reduced for endo-..beta..-glucanase to 0.02 and for ..beta..-glucosidase to 0.005 at 20 degrees C in a phase system of Dextran 40-PEG 40000 in the presence of excess cellulose. At 50 degrees C, K values were increased by a factor of two. In a phase system based on inexpensive crude dextran and PEG, the partition coefficient for endo-..beta..-glucanase was 0.16 and for beta-glucosidase was 0.14 at 20 degrees C with excess cellulose present.

• Research Organization: Univ. of Lund, Sweden
• OSTI ID: 6335463
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 27:7; ISSN BIBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AQUEOUS SOLUTIONS
BIOCONVERSION
CARBOHYDRATES
CELLULASE
CELLULOSE
CHEMICAL REACTIONS
CONTINUOUS CULTURE
DECOMPOSITION
DILUTION
DISPERSIONS
ENZYMATIC HYDROLYSIS
ENZYMES
FRACTIONATION
FUNGI
GLUCOSIDASE
GLYCOSYL HYDROLASES
HYDROLASES
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LYSIS
MIXTURES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SEPARATION PROCESSES
SOLUTIONS
SOLVOLYSIS
SUBSTRATES
TRICHODERMA
TRICHODERMA VIRIDE