Bioassay-directed fractionation of organic contaminants in an estuarine sediment using the new mutagenic bioassay, mutatox (trade name)
Bioassay-directed fractionation of organic compounds was performed on an organic solvent extract of a contaminated estuarine sediment from Black Rock Harbor, Connecticut, using the new mutagenic bioassay, Mutatox. Chemical fractionation methods of the sediment extract included silica-gel-column chromatography (SGCC), followed by C-18 reverse phase HPLC. Compound identification was performed using GC-MS. Mutatox analyses indicated that four of the eight HPLC fractions contained mutagenic or epigenetic compounds. GC-MS analyses detected known mutagenic polycyclic aromatic hydrocarbons (PAHs) in two of the four mutagenic fractions. GC-MS analyses of the other two mutagenic fractions (one operational blank and one sediment fraction) showed no known mutagenic compounds. SGCC and HPLC, combined with the Mutatox bioassay, were useful in reducing both the number and the complexity of fractions analyzed by GC-MS.
- Research Organization:
- Environmental Protection Agency, Narragansett, RI (United States). Environmental Research Lab.
- OSTI ID:
- 6334442
- Report Number(s):
- PB-93-194504/XAB; EPA--600/J-93/183
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
540350* -- Environment
Aquatic-- Site Resource & Use Studies-- (1990-)
ADSORBENTS
AROMATICS
BIOASSAY
CHEMICAL ANALYSIS
CHEMISTRY
CHROMATOGRAPHY
CONNECTICUT
CONTAMINATION
DATA
DEVELOPED COUNTRIES
FRACTIONATION
HYDROCARBONS
INFORMATION
MASS SPECTROSCOPY
MUTAGENS
NORTH AMERICA
NUMERICAL DATA
ORGANIC COMPOUNDS
POLLUTION
POLYCYCLIC AROMATIC HYDROCARBONS
SEDIMENTS
SEPARATION PROCESSES
SILICA GEL
SPECTROSCOPY
STATISTICAL DATA
TOXICITY
TRADE
USA
WATER POLLUTION