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Seasonal dynamics, alkaline phosphatase activity and phosphate uptake of adnate and loosely attached epiphytes in an oligotrophic lake

Technical Report ·
OSTI ID:6333246
This research characterizes the seasonal dynamics of algal epiphytes throughout an annual cycle and examines potential major sources of algal phosphorus. In a phosphorus-limited hardware lake, epiphytic microflora consisted almost entirely of diatoms and blue-green algae, and community structure fluctuated temporally and spatially (with age of macrophyte tissue). Epiphytic ATP was greatest during initial colonization of new macrophyte ramets, and was not correlated with algal biomass except on oldest leaf tissue. Epiphytic chlorophyll was determined primarily by blue-green algal biomass, except on oldest leaf tissue and during colder periods. Epiphytic alkaline phosphatase activity on new and aging macrophyte leaves was significantly less than that on artificial plants, indicating that algae on natural plants were less phosphorus-limited and had obtained a portion of their phosphorus from the macrophytes. Collaborative research quantified the importance of macrophytes as a phosphorus source for algal epiphytes. The author examined the importance of position in determining nutrient availability within the epiphyte matrix. Following short-term assays, scanning electron microscopy (SEM)- and LM-autoradiography were used to directly compare uptake of /sup 33/P-phosphate from the water by intact adnate vs. loosely attached algae, respectively. Loosely attached algae took up significantly more phosphate from the water than did underlying adnate cells, after long-term community acclimation to either phosphorus-poor or phosphorus-enriched conditions.
Research Organization:
Michigan State Univ., East Lansing (USA). Dept. of Botany and Plant Pathology
DOE Contract Number:
FG02-87ER60515
OSTI ID:
6333246
Report Number(s):
DOE/ER/60515-T1-Pt.3; COO-1599-308-Pt.3; ON: TI87012143
Country of Publication:
United States
Language:
English

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Related Subjects

520100* -- Environment
Aquatic-- Basic Studies-- (-1989)
54 ENVIRONMENTAL SCIENCES
550501 -- Metabolism-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALGAE
ALKALINE PHOSPHATASE
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOMASS
CARBOXYLIC ACIDS
CHLOROPHYLL
DAYS LIVING RADIOISOTOPES
DYNAMIC FUNCTION STUDIES
ENERGY SOURCES
ENZYMES
ESTERASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LAKES
LIGHT NUCLEI
METABOLISM
NUCLEI
NUTRIENTS
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATASES
PHOSPHATES
PHOSPHORUS 33
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PHYTOCHROMES
PIGMENTS
PLANTS
PORPHYRINS
PROTEINS
RADIOISOTOPES
RECYCLING
RENEWABLE ENERGY SOURCES
SEASONAL VARIATIONS
SURFACE WATERS
TRACER TECHNIQUES
VARIATIONS