Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Stimulation of allogeneic lymphocytes by skin epidermal cells in the rat. [X radiation, mitomycin C]

Journal Article · · Transplantation; (United States)
OSTI ID:6333193
;
The ability of skin epidermal cells to induce allogeneic lymphocytes into proliferation was examined in mixed skin cell-lymphocyte culture reaction (MSLR). The stimulatng capacity of skin cells was reduced significantly by trypsin digestion, although the damage was repaired by incubation at 37 C for 3 hr. The optimal concentration of mitomycin C for treatment of stimulating cells in the MSLR differed from that in mixed lymphocyte culture reaction (MLR). Irradiation rendered them three to four times more stimulatory than did mitomycin C. Removal of adherent cells from responding cells by passage through a nylon-wool column gave a substantial elevation of the MSLR. The lymphocytes cocultured with skin cells in the primary MSLR incorporated /sup 3/H-thymidine, with the peak at the 6th day of culture. If the lymphocytes primed in the MSLR were restimulated with skin cells from the same stimulating strain, the primed lymphocytes responded promptly and in great magnitude.
Research Organization:
State Univ. of New York, Brooklyn
OSTI ID:
6333193
Journal Information:
Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 27:3; ISSN TRPLA
Country of Publication:
United States
Language:
English

Similar Records

Comparison of $sup 137$Cs irradiation and mitomycin C treatment of stimulator cells in the mixed lymphocyte culture reaction
Journal Article · Thu Aug 01 00:00:00 EDT 1974 · Cell. Immunol., v. 13, no. 2, pp. 326-330 · OSTI ID:4176883
Comparison of /sup 137/Cs irradiation and mitomycin C treatment of stimulator cells in the mixed lymphocyte culture reaction
Journal Article · Thu Aug 01 00:00:00 EDT 1974 · Cell. Immunol.; (United States) · OSTI ID:7349047
Immunoglobulin production in human mixed lymphocyte cultures: implications for co-cultures of cells from patients and healthy donors
Journal Article · Sun Jan 31 23:00:00 EST 1982 · J. Immunol.; (United States) · OSTI ID:5637234

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
EPIDERMIS
EPITHELIUM
IMMUNE REACTIONS
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MITOMYCIN
ORGANS
RADIATION EFFECTS
RADIATIONS
RATS
RODENTS
SKIN
SOMATIC CELLS
STIMULATION
TISSUES
VERTEBRATES
X RADIATION