Prostaglandin F2 alpha stimulates phosphatidylinositol 4,5-bisphosphate hydrolysis and mobilizes intracellular Ca2+ in bovine luteal cells

Journal Article · Mon Jun 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.11.3728· OSTI ID:6332743

Davis, J S; Weakland, L L; Weiland, D A; Farese, R V; West, L A

The present studies were conducted to determine whether prostaglandin F2 alpha (PGF2 alpha) stimulates the production of ''second messengers'' derived from inositol phospholipid hydrolysis and increases intracellular free Ca2+ ((Ca2+)i) in isolated bovine luteal cells. PGF2 alpha provoked rapid (10 sec) and sustained (up to 60 min) increases in the levels of inositol mono-, bis-, and trisphosphates (InsP, InsP2, and InsP3, respectively). InsP3 was formed more rapidly than InsP2 or InsP after PGF2 alpha treatment. In addition, PGF2 alpha increased inositol phospholipid turnover, as evidenced by increased /sup 32/PO/sub 4/ incorporation into phosphatidic acid and phosphatidylinositol. LiCl (1-20 mM) enhanced inositol phosphate accumulation in response to PGF2 alpha. Maximal increases in InsP3 occurred at 1 microM PGF2 alpha, with half-maximal stimulation occurring at 36 nM. The acute effects of PGF2 alpha on InsP3 levels were independent of reductions in extracellular calcium. Prostaglandins E1 and E2 also stimulated increases in inositol phosphate levels, albeit to a lesser extent. PGF2 alpha also induced rapid and concentration-dependent increases in (Ca2+)i as measured by quin-2 fluorescence. The PGF2 alpha-induced increases in (Ca2+)i were maximal within 30 sec (approximately 2- to 3-fold), and (Ca2+)i remained elevated for 8-10 min. The PGF2 alpha-induced increases in (Ca2+)i were also independent of extracellular calcium. These findings demonstrate that the action of PGF2 alpha is coupled to the phospholipase C-InsP3 and diacylglycerol second messenger system in the corpus luteum.

• Research Organization: James A. Haley Veterans Administration Hospital, Tampa, FL
• OSTI ID: 6332743
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 11; ISSN PNASA
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CALCIUM COMPOUNDS
CARBOHYDRATES
CATTLE
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ESTERS
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPIDS
MAMMALS
MEMBRANE TRANSPORT
METABOLISM
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PROSTAGLANDINS
RADIOISOTOPES
REACTION KINETICS
RUMINANTS
SACCHARIDES
TRACER TECHNIQUES
VERTEBRATES