In vitro metabolism of radioactive progesterone and testosterone by the gonads of the protandrous Rhabdosargus sarba at various sexual phases

Journal Article · Thu Aug 01 00:00:00 EDT 1985 · Gen. Comp. Endocrinol.; (United States)

DOI:https://doi.org/10.1016/0016-6480(85)90367-3· OSTI ID:6332713

Yeung, W S; Chan, S T

The in vitro steroidogenic capacity of the gonadal tissue in the protandrous Rhabdosargus sarba was studied. Testicular and ovarian tissues from various sexual phases were used either separately or combined. With progesterone as precursor, high yield of 5 beta-reduced metabolites, and no 11-ketotestosterone or 11 beta-hydroxytestosterone were found. The production of 5 beta-pregnan-3 alpha-ol-20-one and 5 beta-pregnane-3,20-dione was very high in incubations with testicular tissue from intersexes or males and was low in those with ovarian tissue only. The production of 17 alpha-hydroxyprogesterone was high in the female but was low in other sexual phases. With testosterone as precursor, 11-ketotestoterone and 11 beta-hydroxytestosterone were identified. 5 beta-Reductase activity was high in the male and relatively low in the female. 5 alpha-Reduced products, however, were absent. There was an increase in the production of androstenedione as the animal underwent sex reversal. The significance of this change in steroidogenesis in this protandrous fish is at present under investigation. Experimental results also indicated that in the intersexual gonad there may be interaction between the testicular component and the ovarian component in steroidgenesis.

• Research Organization: Univ. of Hong Kong
• OSTI ID: 6332713
• Journal Information: Gen. Comp. Endocrinol.; (United States), Journal Name: Gen. Comp. Endocrinol.; (United States) Vol. 2; ISSN GCENA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANDROGENS
ANDROSTANES
ANIMALS
AQUATIC ORGANISMS
CARBON 14 COMPOUNDS
FISHES
GERM CELLS
GONADS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
METABOLISM
OOCYTES
ORGANIC COMPOUNDS
PHYSIOLOGY
PREGNANES
PROGESTERONE
REPRODUCTION
STEROID HORMONES
STEROIDS
TESTOSTERONE
TRACER TECHNIQUES
VERTEBRATES