Kinetics of the flash-induced P515 response in relation to the H+-permeability of the membrane bound ATPase in spinach chloroplasts
The effect of dicyclohexylcarbodiimide (DCCD) on the kinetics of the flash-induced P515 response and on the activity of the ATPase was investigated in isolated spinach chloroplasts. It was found that after the addition of 5 X 10(-8)mol DCCD the rate of ATP hydrolysis induced by a period of 60 sec illumination was decreased to less than 5% of its original value. At this concentration, hardly any effect, if at all, could be detected on the kinetics of the flash-induced P515 response, neither in dark-adapted nor in light-activated chloroplasts. It was concluded that the presence of concentrations of DCCD, sufficiently high to affect the ATPase activity, does not affect the kinetics of the flash-induced P515 response. Since DCCD decreases the H+ permeability of the membrane-bound ATPase, it was concluded that this permeability coefficient for protons is not an important factor in the regulation of the flash-induced membrane potential and, therefore, does not affect the kinetics of the flash-induced P515 response.
- Research Organization:
- Agricultural Univ., Wageningen, Netherland
- OSTI ID:
- 6331417
- Journal Information:
- J. Bioenerg. Biomembr.; (United States), Vol. 4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ATP-ASE
ENZYME ACTIVITY
CELL MEMBRANES
PERMEABILITY
IMIDES
BIOLOGICAL EFFECTS
ATP
BIOCHEMICAL REACTION KINETICS
CATIONS
CHLOROPHYLL
CHLOROPLASTS
HYDROGEN COMPOUNDS
HYDROLYSIS
PHOTOSYNTHESIS
PROTONS
VEGETABLES
ACID ANHYDRASES
BARYONS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHARGED PARTICLES
CHEMICAL REACTIONS
DECOMPOSITION
ELEMENTARY PARTICLES
ENZYMES
FERMIONS
FOOD
HADRONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROLASES
IONS
KINETICS
LYSIS
MEMBRANES
NUCLEONS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHOHYDROLASES
PHOTOCHEMICAL REACTIONS
PHYTOCHROMES
PIGMENTS
PLANTS
PORPHYRINS
REACTION KINETICS
SOLVOLYSIS
SYNTHESIS
560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)