Ligninolytic enzyme system of Phanerochaete chrysosporium: synthesized in the absence of lignin in response to nitrogen starvation

Journal Article · Fri Sep 01 04:00:00 EDT 1978 · J. Bacteriol.; (United States)

OSTI ID:6327665

Keyser, P; Kirk, T K; Zeikus, J G

The relationship between growth, nutrient nitrogen assimilation, and the appearance of ligninolytic activity was examined in stationary batch cultures of the wood-destroying hymenomycete Phanerochaete chrysosporium Burds. grown under conditions optimized for lignin metabolism. A reproducible sequence of events followed inoculation: 0 to 24 h, germination, linear growth, and depletion of nutrient nitrogen; 24 to 48 h, cessation of linear growth and derepression of ammonium permease activity (demonstrating nitrogen starvation); 72 to 96 h, appearance of ligninolytic activity (synthetic /sup 14/C-lignin ..-->.. /sup 14/CO/sub 2/). Experiments with cycloheximide demonstrated that appearance of ligninolytic activity occurs irrespective of the presence of lignin; lignin did not induce additional activity. Addition of NH/sub 4//sup +/ to cultures immediately prior to the time of appearance of the ligninolytic system delayed its appearance, suggesting that the NH/sub 4//sup +/ led to interference with synthesis of the enzyme system. Addition of NH/sub 4//sup +/ to ligninolytic cultures resulted in an eventual, temporary decrease in ligninolytic activity. The results suggest that all or essential protein components of the ligniolytic enzyme system are synthesized as part of a series of physiological (secondary metabolic) events that are initiated by nutrient nitrogen starvation.

• Research Organization: Forest Service, Madison, WI
• OSTI ID: 6327665
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 135:3; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
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550200 -- Biochemistry
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBOHYDRATES
CRYOGENIC FLUIDS
CULTURE MEDIA
ELEMENTS
ENZYMES
FLUIDS
FUNGI
GROWTH
KINETICS
LIGNIN
METABOLISM
NITROGEN
NONMETALS
NUTRIENTS
ORGANIC COMPOUNDS
PHANEROCHAETE
PLANTS
POLYSACCHARIDES
REACTION KINETICS
SACCHARIDES
SYNTHESIS