The reserpinized rat in the study of cystic fibrosis: x-ray microanalysis of submandibular gland and pancreas
The chronically reserpinized rat has been suggested as an animal model for cystic fibrosis. X-ray microanalysis of thick and thin cryosections was carried out to assess elemental redistribution in the submandibular glands and the pancreas of reserpinized rats at the cellular and subcellular level. In the submandibular gland of reserpinized rats, calcium and magnesium concentrations were significantly elevated. Mucus globules, secretory granules, and endoplasmic reticulum were the primary sites of the localization of excess calcium and magnesium. A significant potassium loss from the gland had occurred, particularly from the serous cells. Electron microscopy of conventionally prepared tissue showed marked swelling of the endoplasmic reticulum, especially in mucous cells. The elemental changes in the pancreatic acinar cells of reserpinized rats were reminiscent of elemental redistribution connected with cell death: increased levels of sodium, chlorine, and calcium and decreased levels of magnesium and potassium. Ultrastructural changes included swelling of the endoplasmic reticulum and obstruction of the acinar lumen. It is concluded tha elemental redistribution in chronically reserpinized rats presents interesting parallels with cystic fibrosis.
- Research Organization:
- Wenner-Gren Institute, University of Stockholm, Sweden
- OSTI ID:
- 6326222
- Journal Information:
- Ultrastruct. Pathol.; (United States), Vol. 3:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
FIBROSIS
BIOLOGICAL MODELS
PATHOLOGY
CALCIUM
ENDOPLASMIC RETICULUM
MAGNESIUM
PANCREAS
POTASSIUM
RATS
ULTRASTRUCTURAL CHANGES
X-RAY EMISSION ANALYSIS
ALKALI METALS
ALKALINE EARTH METALS
ANIMALS
BODY
CELL CONSTITUENTS
CHEMICAL ANALYSIS
DIGESTIVE SYSTEM
ELEMENTS
ENDOCRINE GLANDS
GLANDS
MAMMALS
METALS
MORPHOLOGICAL CHANGES
NONDESTRUCTIVE ANALYSIS
ORGANOIDS
ORGANS
PATHOLOGICAL CHANGES
RODENTS
VERTEBRATES
550900* - Pathology
550602 - Medicine- External Radiation in Diagnostics- (1980-)