Restriction fragment length polymorphisms associated with substance P gene

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6325776

de Miguel, C; Bonner, T; Detera-Wadleigh, S

Substance P (SP) is an important neuropepetide detected in a variety of locations in the central nervous system. Variations in SP content or SP receptors in psychiatric disorders have been described. Using SP clones as probes the authors have found three restriction fragment length polymorphisms (RFLPs) in the SP gene. The RFLPs are generated by digestion of genomic DNA with the MspI, and RsaI and NcoI restriction endonucleases. The MspI RFLP is detected by two genomic clones mapping to the 5' end of the gene while the RsaI and NcoI rFLPs are both detected by two genomic clones on the 3' end and also by a full-length cDNA clone of the gene. All three RFLPs are characterized by two alleles. For the MspI RFLP the frequency of both alleles is similar, for the Rsa I and NcoI RFLP one of the alleles is significantly more abundant than the other. These RFLPs are now being used to determine whether any of the alleles correlate with either schizophrenia or affective disorder.

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 6325776
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
550900 -- Pathology
59 BASIC BIOLOGICAL SCIENCES
CENTRAL NERVOUS SYSTEM
CLONING
DNA
DNA-ASE
ENDONUCLEASES
ENZYMES
ESTERASES
GENES
GENETIC MAPPING
HYDROLASES
ISOENZYMES
MAPPING
MEMBRANE PROTEINS
MENTAL DISORDERS
NERVOUS SYSTEM
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
PEPTIDES
PHOSPHODIESTERASES
PROTEINS
RECEPTORS