Chemotactic behavior of deep subsurface bacteria toward carbohydrates, amino acids and a chlorinated alkene
- Puerto Rico Univ., Rio Piedras (Puerto Rico). Dept. of Biology
The chemotactic behavior of deep terrestrial subsurface bacteria toward amino acids, carbohydrates and trichloroethylene was assayed using a modification of the capillary method and bacterial enumeration by acridine orange direct counts. Eleven isolates of bacteria isolated from six different geological formations were investigated. A bimodal response rather than an absolute positive or negative response was observed in most assays. Most of the isolates were positively chemotactic to low concentrations of substrates and were repelled by high concentrations of the same substrate. However, this was not the case for trichloroethylene (TCE) which was mostly an attractant and elicited the highest responses in all the isolates when compared with amino acids and carbohydrates. The movement rates of these isolates in aseptic subsurface sediments in the absence and presence of TCE were also determined using a laboratory model. All of the isolates showed distinct response range, peak, and threshold concentrations when exposed to the same substrates suggesting that they are possibly different species as has been inferred from DNA homology studies. 101 refs., 4 figs., 57 tabs.
- Research Organization:
- Savannah River Lab., Aiken, SC (USA)
- Sponsoring Organization:
- DOE/DP; DOHHS
- DOE Contract Number:
- AC09-76SR00001
- OSTI ID:
- 6324569
- Report Number(s):
- DP-1779; ON: DE91002548; CNN: RR-8102
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
540220* -- Environment
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550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALKENES
AMINO ACIDS
ANAEROBIC CONDITIONS
AQUIFERS
BACTERIA
BIODEGRADATION
BOREHOLES
CARBOHYDRATES
CARBOXYLIC ACIDS
CAVITIES
CHEMICAL REACTIONS
CHEMICAL WASTES
CHLORINATED ALIPHATIC HYDROCARBONS
DECOMPOSITION
DISSOLUTION
ENVIRONMENTAL IMPACTS
ENVIRONMENTAL TRANSPORT
GEOLOGIC FORMATIONS
GROUND WATER
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROCARBONS
HYDROGEN COMPOUNDS
LEACHING
MASS TRANSFER
MATERIALS
MICROORGANISMS
MORPHOLOGICAL CHANGES
NATIONAL ORGANIZATIONS
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN COMPOUNDS
POLLUTANTS
POLLUTION
SAVANNAH RIVER PLANT
SEDIMENTS
SEPARATION PROCESSES
SUBSURFACE ENVIRONMENTS
TOXIC MATERIALS
US AEC
US DOE
US ERDA
US ORGANIZATIONS
WASTES
WATER