Separation of CFU-S from primitive cells responsible for reconstitution of the bone marrow hemopoietic stem cell compartment following irradiation: evidence for a pre-CFU-S cell

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Exp. Hematol.; (United States)

OSTI ID:6324331

Ploemacher, R E; Brons, R H

We have studied the in vivo spleen colony-forming ability and marrow repopulating ability of murine bone marrow cells differing in mitochondrial activity. Following centrifugal elutriation the cells were sorted on the basis of rhodamine-123 fluorescence intensity within a predetermined light scatter window. It is shown that a class of hemopoietic stem cells exists that differs from the majority of day-12 spleen colony-forming units (CFU-S) in that it has low mitochondrial activity and a high capacity to generate in time new day-12 CFU-S and cells that rescue recipients from radiation-inflicted death. These data add direct evidence for the identity of pre-CFU-S and CFU-S by physical separation.

• Research Organization: Erasmus Univ., Rotterdam (Netherlands)
• OSTI ID: 6324331
• Journal Information: Exp. Hematol.; (United States), Journal Name: Exp. Hematol.; (United States) Vol. 17:3; ISSN EXHMA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
ANIMALS
BONE MARROW CELLS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL FLOW SYSTEMS
CENTRIFUGATION
CHIMERAS
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DYES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LIGHT SCATTERING
MAMMALS
MICE
MITOCHONDRIA
MOSAICISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
RADIATION CHIMERAS
REAGENTS
RHODAMINES
RODENTS
SCATTERING
SEPARATION PROCESSES
SOMATIC CELLS
SPLEEN CELLS
STEM CELLS
ULTRACENTRIFUGATION
VERTEBRATES