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The examination and quantitation of tissue cytosolic receptors for 2,3,7,8-tetrachlorodibenzo-p-dioxin using hydroxylapatite

Journal Article · · Anal. Biochem.; (United States)
 [1];
  1. Univ. of Rochester, NY
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An assay using hydroxylapatite has been developed for the examination and quantitation of cytosolic receptors for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). This method, as compared to others, has a relatively high ratio of specific to nonspecific binding of (/sup 3/H)TCDD and is relatively rapid. The total number of specific binding sites and equilibrium dissociation constants (K/sub D/) for (/sup 3/H)TCDD were determined in hepatic cytosol from Sprague-Dawley rats and C57BL/6J, DBA/2J, and B6D2F/sub 1//J mice. With the exception of the cytosol from DBA/2J mice in which little specific binding was observed, high-affinity, specific binding was obtained in all cases. The rat hepatic cytosol demonstrated the highest affinity for (/sup 3/H)TCDD with a K/sub D/ of 0.12 nM. The total amount of specific (/sup 3/H)TCDD binding in hepatic cytosol from B6D2F/sub 1//J mice was found to be intermediate between that of the C57BL/6J and DBA/2J parents. Ligand competition studies suggest the specificity of binding of various compounds to the receptor species from Sprague-Dawley rats is similar to that observed with the hepatic cytosol from C57BL/6J mice. These data support the hypothesis that, as in mice, the particular cytosol binding species in rats is the receptor that may mediate the induction of aryl hydrocarbon hydroxylase activity.

DOE Contract Number:
AC02-76EV03490
OSTI ID:
6324183
Journal Information:
Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 124:1; ISSN ANBCA
Country of Publication:
United States
Language:
English

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Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
DIOXIN
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROXYLASES
KINETICS
LABELLED COMPOUNDS
LIGANDS
LIVER CELLS
MAMMALS
MICE
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SOMATIC CELLS
TRITIUM COMPOUNDS
VERTEBRATES