Monochromosomal hybrid cell assay for evaluating the genotoxicity of environmental chemicals
The development and utilization of a monochromosomal hybrid cell assay for detecting aneuploidy and chromosomal aberrations are described. The monochromosomal hybrid cell lines were produced by a two-step process involving transfer of a marker bacterial gene to a human chromosome and then by integration of that human chromosome into a mouse complement of chromosomes through microcell fusion. For chemically induced aneuploidy, the segregation of a single human chromosome among mouse chromosomes is used as a cytogenetic marker. The genetic assay for aneuploidy is based on the ability of the cells to grow in a medium that selects for the loss of the human chromosome. The assay for clastogenicity is based on survival of the cells after treatment with the chemicals in medium that selects for retention of the human chromosome but loss of its segment containing diphtheria toxin locus. The assays greatly simplify the detection of chromosomal aberrations induced by environmental factors at low-dose levels.
- Research Organization:
- Environmental Protection Agency, Research Triangle Park, NC (USA)
- OSTI ID:
- 6323574
- Journal Information:
- Cell Biol. Toxicol.; (United States), Vol. 4:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
XENOBIOTICS
GENETIC EFFECTS
ANEUPLOIDY
BACTERIA
BIOASSAY
CHROMOSOMAL ABERRATIONS
ENVIRONMENTAL EXPOSURE
GENETIC ENGINEERING
MAN
MICE
TOXICITY
ANIMALS
BIOLOGICAL EFFECTS
MAMMALS
MICROORGANISMS
MUTATIONS
PLOIDY
PRIMATES
RODENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)