Isolation and characterization of Schwanniomyces alluvius amylolytic enzymes

Journal Article · Sun Aug 01 04:00:00 EDT 1982 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6323449

Wilson, J J; Ingledew, W M

The extracellular amylolytic enzymes of Schwanniomyces alluvius were studied to determine future optimization of this yeast for the production of industrial ethanol from starch. Both alpha-amylase and glucoamylase were isolated and purified. Alpha-amylase has an optimum pH of 6.3 and was stable from pH 4.5 to 7.5. The optimum temperature for the enzyme was 40 degrees C, but it was quickly inactivated at temperatures above 40 degrees C. The Km for soluble starch was 0.364 mg/ml. The molecular weight was calculated to be 61,900 + or - 700. Alpha-amylase was capable of releasing glucose from starch, but not from pullulan. Glucoamylase had an optimum pH of 5.0 and was stable from pH 4.0 to more than 8.0. The optimum temperature for the enzyme was 50 degrees C, and although less heat sensitive than alpha-amylase, it was quickly inactivated at 60 degrees C. Km values were 12.67 mg/ml for soluble starch and 0.72 mM for maltose. The molecular weight was calculated to be 155,000 + or - 3,000. Glucoamylase released only glucose from both soluble starch and pullulan. Schwanniomyces alluvius is one of the very few yeasts to possess both alpha-amylase and glucoamylase as well as some fermentative capacity to produce ethanol. (Refs. 9).

• Research Organization: Agricultural Microbiology Section, Dept of Dairy and Food Science, Univ of Saskatchewan, Saskatoon, Saskatchewan Canada S7N OWO
• OSTI ID: 6323449
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 44:2; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
ALCOHOLS
ALDEHYDES
AMYLASE
BIOCONVERSION
BIOSYNTHESIS
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYME ACTIVITY
ENZYMES
ETHANOL
FERMENTATION
FUNGI
GLUCOSE
GLUCOSIDASE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
HYDROXY COMPOUNDS
LYSIS
MICROORGANISMS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
REAGENTS
SACCHARIDES
SACCHARIFICATION
SOLVOLYSIS
STARCH
SYNTHESIS
YEASTS