Analysis of binding of a technetium-99m-labeled monoclonal antibody to lentivirus-infected cells

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Investigative Radiology; (USA)

DOI:https://doi.org/10.1097/00004424-199011000-00006· OSTI ID:6322424

Papageorges, M; Gavin, P R; Adams, D S; Cheevers, W P; Barbee, D D; Sande, R D ^[1]

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1. Washington State Univ., Pullman (USA)

Caprine arthritis-encephalitis (CAE) is a model for the study of lentiviral infections. The authors' hypothesis is that radioimmunodetection has the potential to detect lentiviral proteins at the surface of infected cells. A monoclonal antibody (CAEV92A1) specific for a CAE virus (CAEV)-associated glycoprotein and a control antibody were radiolabeled with technetium-99m ({sup 99m}Tc) using the pretinning method. Cell binding assays were used to evaluate immunoreactivity and binding properties of {sup 99m}Tc-labeled antibodies to CAEV-infected cells. {sup 99m}Tc-CAEV92A1 bound preferentially to paraformaldehyde-fixed and live CAEV-infected cells. {sup 99m}Tc-CAEV92A1 did not appear to be shed rapidly from its binding site.

• OSTI ID: 6322424
• Journal Information: Investigative Radiology; (USA), Journal Name: Investigative Radiology; (USA) Vol. 25:11; ISSN INVRA; ISSN 0020-9996
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
COUNTING TECHNIQUES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
GLYCOPROTEINS
HOURS LIVING RADIOISOTOPES
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MEMBRANE PROTEINS
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOIMMUNODETECTION
RADIOIMMUNOSCINTIGRAPHY
RADIOISOTOPE SCANNING
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SCINTISCANNING
SPECIFICITY
TECHNETIUM 99
TECHNETIUM ISOTOPES
TRACER TECHNIQUES
VIRAL DISEASES
YEARS LIVING RADIOISOTOPES