Preferential differentiation of murine CFU-S toward granulopoiesis and megakaryocytopoiesis after in vitro incubation of bone marrow with ASTA-Z 7557

Journal Article · Wed Jul 01 04:00:00 EDT 1987 · Exp. Hematol.; (United States)

OSTI ID:6321978

Sainteny, F; Lopez, M; Mary, J Y; Frindel, E

We investigated the in vitro effect of ASTA-Z 7557 on the qualitative aspects of murine CFU-S differentiation, as assessed by the histological nature of day-9 colonies generated in the spleen of irradiated mice by bone marrow exposed to the drug at concentrations ranging from 0 to 150 micrograms/ml. The proportion of erythrocytic colonies declined linearly with the logarithm of the dose (a 22% decrease per log), whereas the granulocytic and megakaryocytic colony proportions increased linearly (a 10% increase per log for both cell lineages). This suggests a preferential channeling of CFU-S differentiation toward granulopoietic and megakaryocytic cell lineages as a consequence of the in vitro chemotherapy, and supports the hypothesis that some alteration of the qualitative potential of CFU-S to differentiate after in vitro purging of bone marrow with ASTA-Z 7557 takes place prior to autologous bone marrow transplantation.

• Research Organization: INSERM U250, Gustave-Roussy Institute, Villejuif, France
• OSTI ID: 6321978
• Journal Information: Exp. Hematol.; (United States), Journal Name: Exp. Hematol.; (United States) Vol. 15:6; ISSN EXHMA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BLOOD FORMATION
BODY
BONE MARROW
CELL DIFFERENTIATION
COLONY FORMATION
DOSE-RESPONSE RELATIONSHIPS
DRUGS
HEMATOPOIETIC SYSTEM
IN VITRO
IRRADIATION
MAMMALS
MICE
ORGANS
RODENTS
SOMATIC CELLS
SPLEEN
STEM CELLS
TISSUES
TRANSPLANTS
VERTEBRATES