Two-phase flow cell for chemiluminescence and bioluminescence measurements

Journal Article · Sun Jan 01 04:00:00 EST 1984 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00270a043· OSTI ID:6321650

Mullin, J L; Seitz, W R

A new approach to two-phase CL (chemiluminescence) measurements is reported. A magnetically stirred reagent phase is separated from the analyte phase by a dialysis membrane so that only smaller molecules can go from one phase to the other. The system is designed so that the analyte phase flows through a spiral groove on an aluminum block that is flush against the dialysis membrane. As solution flows through the spiral grove, analyte diffuses into the reagent phase where it reacts to produce light. A simple model is developed to predict how this system will behave. Experimentally, the system is evaluated by using the luminol reaction catalyzed by peroxidase, the firefly reaction, and the bacterial bioluminescence reaction. 10 references, 4 tables, 6 figures.

• Research Organization: Univ. of New Hampshire, Durham
• OSTI ID: 6321650
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 56:6; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400104* -- Spectral Procedures-- (-1987)
AMINES
AZINES
BIOLUMINESCENCE
CHEMICAL ANALYSIS
CHEMILUMINESCENCE
DATA
DIALYSIS
EXPERIMENTAL DATA
FLOW MODELS
HETEROCYCLIC COMPOUNDS
INFORMATION
LUMINESCENCE
LUMINOL
MATHEMATICAL MODELS
MEMBRANES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHTHALAZINES
PYRIDAZINES
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES