Yolk sac endoderm: exclusive site of serum protein synthesis in the early chick embryo
Journal Article
·
· Dev. Biol.; (United States)
In order to determine which cell type or types synthesized serum proteins, yolk sacs from 4-day chick embryos were manually separated into ectoderm, mesoderm, and endoderm and incubated in the presence of radioactive valine. Analysis of the incubation media by polyacrylamide gel electrophoresis as well as by immunoprecipitation showed that all serum proteins were synthesized exclusively by the cells of the endoderm. These included transferrin and three embryo-specific serum proteins: ..cap alpha..reverse arrowglobulin ..cap alpha..-globulin b, and prealbumin.
- Research Organization:
- Univ. of Connecticut, Storrs
- OSTI ID:
- 6319119
- Journal Information:
- Dev. Biol.; (United States), Journal Name: Dev. Biol.; (United States) Vol. 75; ISSN DEBIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
AMINO ACIDS
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CULTURES
CULTURE MEDIA
DYNAMIC FUNCTION STUDIES
EGGS
EMBRYONIC CELLS
EMBRYOS
GLOBULINS
GLOBULINS-ALPHA
GLOBULINS-BETA
INCUBATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
METALLOPROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
SYNTHESIS
TRACER TECHNIQUES
TRANSFERRIN
VALINE
550301 -- Cytology-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
AMINO ACIDS
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CULTURES
CULTURE MEDIA
DYNAMIC FUNCTION STUDIES
EGGS
EMBRYONIC CELLS
EMBRYOS
GLOBULINS
GLOBULINS-ALPHA
GLOBULINS-BETA
INCUBATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
METALLOPROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
SYNTHESIS
TRACER TECHNIQUES
TRANSFERRIN
VALINE