Ethanol-induced phosphorylation of cytokeratin in cultured hepatocytes
- Univ. of Ottawa, Ontario (Canada)
The authors studied the effect of ethanol on the phosphorylation of cytokeratins (CKs) in cultured hepatocytes since CK filaments are resulted by phosphorylation and they are abnormal in alcoholic liver disease. Hepatocytes were obtained from 14-day-old rats and cultured for 48 hrs. The hepatocytes were exposed to ethanol for 30 min. The residual insoluble cytoskeletons were analyzed by two-dimensional gel electrophoresis and autoradiography. 2D gel electrophoresis showed CK 55 and CK 49 or 8 and 18 and actin. The CKs had several isoelectric variants. The most basic spot was the dominant protein which was not phosphorylated. The more acidic spots were phosphorylated. After ethanol treatment, the phosphorylation of CK 55 and CK 49 were markedly increased over controls. They compared these results, with the effect of vasopressin, TPA and db-cAMP on the phosphorylation of CKs. Vasopressin and TPA caused the phosphorylation of CK 55 and 49 but db-cAMP did not.
- OSTI ID:
- 6317635
- Journal Information:
- Life Sciences; (USA), Vol. 47:10; ISSN 0024-3205
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ETHANOL
BIOLOGICAL EFFECTS
KERATIN
PHOSPHORYLATION
AMP
AUTORADIOGRAPHY
CELL CULTURES
COMPARATIVE EVALUATIONS
DIGESTIVE SYSTEM DISEASES
LIVER CELLS
PHOSPHOTRANSFERASES
PLASMINOGEN
TWO-DIMENSIONAL ELECTROPHORESIS
VASOPRESSIN
ALCOHOLS
ANIMAL CELLS
CHEMICAL REACTIONS
DISEASES
DRUGS
ELECTROPHORESIS
ENZYMES
FIBRINOLYTIC AGENTS
HEMATOLOGIC AGENTS
HORMONES
HYDROXY COMPOUNDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PITUITARY HORMONES
PROTEINS
SCLEROPROTEINS
SOMATIC CELLS
TRANSFERASES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques