Molecular cloning and structural characterization of the R locus of maize: Annual progress report for period September 29, 1986-September 28, 1987
Last year we reported on the isolation of a molecular clone of the R locus of maize using an Ac transposon tagging strategy. During the past year we have confirmed the identity of this clone and have begun a molecular analysis of several R alleles. Our main focus continues to be on the analysis of R-r, an allele containing both seed (S) and plant (P) components. Genomic blot analysis and gene cloning experiments suggest that the R-r allele may be organized as a triplication. In addition to (P) and (S), there appears to be a third cryptic component we refer to as (Q). We are attempting to clone the complete R-r allele by chromosome walking techniques to determine the molecular organization of R-r. The second objective of our research on R is to understand the mechanism of tissue-specific regulation of anthocyanin. We are characterizing several R alleles that condition different pigmentation patterns in plant and seed tissues. In order to determine the allelic differences among tissue-specific components we have obtained genomic clones and are performing DNA sequence analysis to regions of several tissue-specific components that may be responsible for these allelic differences. 1 ref.
- Research Organization:
- Yale Univ., New Haven, CT (USA). Dept. of Biology
- DOE Contract Number:
- FG02-86ER13627
- OSTI ID:
- 6307154
- Report Number(s):
- DOE/ER/13627-1; ON: DE87014149
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Organization of the R chromosome region in maize
Molecular cloning and structural characterization of the R locus maize: Annual progress report, September 1, 1987--May 31, 1988
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59 BASIC BIOLOGICAL SCIENCES
PIGMENTS
RECOMBINANT DNA
DNA-CLONING
GENE REGULATION
MAIZE
TISSUE DISTRIBUTION
CEREALS
CLONING
DISTRIBUTION
DNA
GRASS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
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