Binding sites for /sup 3/H-LTC4 in membranes from guinea pig ileal longitudinal muscle
Journal Article
·
· Prostaglandins; (United States)
OSTI ID:6306017
Leutriene (LTC4) is one of the components of Slow Reacting Substance of Anaphylaxis (SRS-A) and is a potent constrictor of guinea pig ilea. The contraction is likely to be a receptor-mediated process. Here the authors report the existence of specific binding sites for /sup 3/H-LTC4 in a crude membrane preparation from guinea pig ileal longitudinal muscle. At 4 degrees C in the presence of 20 mM Serine-borate, binding increases linearly with protein concentration, reaches equilibrium in 10 minutes, and is reversible upon addition of 3 x 10(-5) M unlabelled LTC4. The dissociation curve is consistent with the existence of more than one class of binding site. Ca++ and Mg++ greatly enhance the binding of /sup 3/H-LTC4 at equilibrium. In the presence of 5 mM CaCl/sub 2/ and MgCl/sub 2/ not only LTC4 (IC50 10(-7)M), but also LTD4 and the SRS-A antagonist FPL 55712 can compete with /sup 3/H-LTC4 for its binding sites. FPL 55712 only displaces 60-70% of the total amount bound, while LTC4 displaces 90-95%. These studies indicate that multiple classes of binding sites exist for /sup 3/H-LTC4 in guinea pig ileal longitudinal muscle, and that at least part of these binding sites might be related to the ability of LTC4 to contract guinea pig ilea.
- Research Organization:
- Univ. of Milan, Italy
- OSTI ID:
- 6306017
- Journal Information:
- Prostaglandins; (United States), Journal Name: Prostaglandins; (United States) Vol. 27:3; ISSN PRGLB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CALCIUM CHLORIDES
CALCIUM COMPOUNDS
CALCIUM HALIDES
CELL CONSTITUENTS
CELL MEMBRANES
CHLORIDES
CHLORINE COMPOUNDS
CONTRACTION
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
GUINEA PIGS
HALIDES
HALOGEN COMPOUNDS
INTESTINES
KINETICS
LABELLED COMPOUNDS
MAGNESIUM CHLORIDES
MAGNESIUM COMPOUNDS
MAMMALS
MEMBRANES
MUSCLES
ORGANS
PROSTAGLANDINS
REACTION KINETICS
RECEPTORS
RODENTS
SMALL INTESTINE
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CALCIUM CHLORIDES
CALCIUM COMPOUNDS
CALCIUM HALIDES
CELL CONSTITUENTS
CELL MEMBRANES
CHLORIDES
CHLORINE COMPOUNDS
CONTRACTION
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
GUINEA PIGS
HALIDES
HALOGEN COMPOUNDS
INTESTINES
KINETICS
LABELLED COMPOUNDS
MAGNESIUM CHLORIDES
MAGNESIUM COMPOUNDS
MAMMALS
MEMBRANES
MUSCLES
ORGANS
PROSTAGLANDINS
REACTION KINETICS
RECEPTORS
RODENTS
SMALL INTESTINE
TRITIUM COMPOUNDS
VERTEBRATES