Ozone response of foliage and cells of sensitive and tolerant potato cultivars

Thesis/Dissertation · Sat Jan 01 04:00:00 EST 1983

OSTI ID:6304502

Illman, B L

The ozone response was contrasted between Cherokee' and Norchip', relatively ozone-sensitive and -tolerant potato cultivars, respectively. Leaf conductance of the two cultivars was determined with a diffusive resistance porometer before, during and after a 3-hour exposure to 0.25 ppm ozone (490 ..mu..g m3). While Cherokee foliage had a higher leaf conductance at the onset of the ozone exposure, conductance of Norchip foliage was too high throughout the experiment to account for relative tolerance of the latter cultivar. Norchip and Cherokee both exhibited abaxial and bifacial necrosis with more numerous lesions on the abaxial surface. Abaxial and bifacial necrosis were characterized by injured spongy, and spongy plus palisade cells, respectively. The percent of injured cell types were the same in both cultivars, but the absolute number of cells injured was greater in Cherokee than Norchip. Two types of cell injury were observed in necrotic lesions. One type of injury was characterized by collapsed, basophilic cells and the other by intact, basophilic cells. The ozone response of isolated leaf protoplasts of the two cultivars was similar. Two types of protoplast injury were observed, one characterized by lysis and the other by reduced staining with fluorescein diacetate. Maximum protoplast response to ozone was observed within the first 5 minutes of a 15 minute exposure. Little additional protoplast response occurred after 10 minutes.

• OSTI ID: 6304502
• Country of Publication: United States
• Language: English

Related Subjects

560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
BIOLOGICAL EFFECTS
COMPARATIVE EVALUATIONS
FOOD
INJURIES
LEAVES
OZONE
PLANTS
POLLUTION
POTATOES
SENSITIVITY
TUBERS
VARIATIONS
VEGETABLES