Genetic effects of microwave exposure on mammalian cells in vitro. Volume 1. Annual report February 1980-June 1981
The effects of radiofrequency radiation (RFR) on the DNA repair process in MRC-5 normal human fibroblast cells grown in vitro have been investigated. The power levels chosen, 1 and 10 mW/sq cm, did not result in measurable temperature above the 37 C incubation temperature at either 350 MHz or 1.2 GHz (continuous or pulse-wave modes). DNA repair was induced by exposure of the cells to ultraviolet light (UV). Repair synthesis was measured by means of a repair replication protocol; i.e., a repair labeling incubation with 3H-thymidine and nonradioactive 5-bromodeoxyuridine (or 3H-BrUdR in early experiments), followed by DNA isolation and two sequential alkali cesium chloride-cesium sulfate density gradient centrifugations. In summary, 1.2-GHz continuous wave (CW) and 350 pulse-wave and continuous-wave RFR, at power levels of 1 and 10 mW/sq cm, did not appear to perturb UV light-induced DNA repair synthesis. A possible stimulation by 350-MHz CW radiation of repair label incorporation during the first hour after UV exposure, at 1 mW/sq cm (but not at 10 mW/sq cm), remains to be confirmed. No evidence exists of RFR induction, by itself, of DNA damage and repair at 1.2 GHz and 350-MHz (CW) at a power level of 10 mW/sq cm.
- Research Organization:
- Texas Univ., San Antonio (USA). Health Science Center
- OSTI ID:
- 6300317
- Report Number(s):
- AD-A-145946/0/XAD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560400* -- Other Environmental Pollutant Effects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CONNECTIVE TISSUE CELLS
DNA REPAIR
ELECTROMAGNETIC RADIATION
FIBROBLASTS
IN VITRO
MAMMALS
MAN
MICROWAVE RADIATION
PRIMATES
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
RESPONSE MODIFYING FACTORS
SOMATIC CELLS
ULTRAVIOLET RADIATION
VERTEBRATES