Metabolism of benzo(a)pyrene by human epithelial and fibroblastic cells: metabolite patterns and DNA adduct formation

Journal Article · Fri Jan 01 04:00:00 EST 1982 · J. Cell. Biochem.; (United States)

DOI:https://doi.org/10.1002/jcb.1982.240180202· OSTI ID:6299944

Bartley, J ^[1]; Bartholomew, J C; Stampfer, M R

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1. Peralta Cancer Research Inst., Oakland, CA

It was demonstrated in cell culture that mammary epithelial cells from normal human breast specimens metabolize benzo(a)pyrene (BaP) and form adducts with the bases of their DNA more readily and at lower concentrations of BaP than do fibroblasts from the same specimens. BaP metabolism and adduct formation was determined in the same incubations with epithelial cells grown out in early passage from each of three specimens and with fibroblasts from one of these specimens. The metabolite pattern of the epithelial cells was indicative of preferential formation of 7, 8-dihydrodiol-9, 10-dihydroepoxybenzo(a)pyrene the ultimate carcinogen. In contrast, fibroblasts formed mainly mono- and dihydroxide derivatives of BaP. The metabolite pattern from epithelial cells was compatible with the ease in which adducts between DNA and the diolepoxide of benzo(a)pyrene were formed. These results provide evidence that chemical carcinogens should be considered as possible factors in the induction of breast cancer in women.

• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6299944
• Journal Information: J. Cell. Biochem.; (United States), Journal Name: J. Cell. Biochem.; (United States) Vol. 18; ISSN JCEBD
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BODY
CELL CULTURES
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DNA
EPITHELIUM
FIBROBLASTS
GLANDS
HYDROCARBONS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAMMARY GLANDS
MAN
METABOLISM
METABOLITES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
SEPARATION PROCESSES
SOMATIC CELLS
TISSUES
VERTEBRATES