/sup 2/H NMR demonstration of amino acid - nucleotide interactions
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
Deuterium (/sup 2/H) NMR was used to investigate the interaction of L-Phenylalanine (with aromatic protons replaced by deuterons (Phe-D/sub 5/)), with 5'-AMP and polyadenylic acid (poly A). A considerable change in line width of the aromatic deuteron signals of Phe was observed. These data were plotted using a Scatchard-type equation, and yielded apparent binding constants for L-Phe to 5'-AMP and poly A of 7 and 11 M/sup -1/, respectively. Future applications of /sup 2/H-NMR in the study of nucleic acid-protein interactions are discussed.
- Research Organization:
- Univ. of Alabama Medical Center, Birmingham
- OSTI ID:
- 6299743
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 106:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
NUCLEIC ACIDS
NMR SPECTRA
PHENYLALANINE
ADENYLIC ACID
AMP
CHEMICAL BONDS
DEUTERIUM
INTERACTIONS
LINE WIDTHS
MONOMERS
PROTEINS
AMINO ACIDS
CARBOXYLIC ACIDS
HYDROGEN ISOTOPES
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
SPECTRA
STABLE ISOTOPES
550201* - Biochemistry- Tracer Techniques
NUCLEIC ACIDS
NMR SPECTRA
PHENYLALANINE
ADENYLIC ACID
AMP
CHEMICAL BONDS
DEUTERIUM
INTERACTIONS
LINE WIDTHS
MONOMERS
PROTEINS
AMINO ACIDS
CARBOXYLIC ACIDS
HYDROGEN ISOTOPES
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
SPECTRA
STABLE ISOTOPES
550201* - Biochemistry- Tracer Techniques