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U.S. Department of Energy
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Peptide F (pro-enkephalin fragment): radioimmunoassay, and stress-induced changes in adrenal

Journal Article · · Life Sci.; (United States)
 [1]; ;
  1. Mental Health Research Inst., Ann Arbor, MI
Utilizing a nine amino-acid (Asp-Glu-Leu-Tyr-Pro-Leu-Glu-Val-Glu) non-enkephalin containing fragment of Peptide F from the pro-enkephalin molecule, a radioimmunoassay was developed. Extraction of bovine, rat, and guinea pig adrenomedullary preparations demonstrated this fragment to be present and apparently partially conserved across species. In rats, acute inescapable foot-shock stress led to a significant decrease of the immunoreactive material in the adrenal medulla. Chronic daily stress for two weeks resulted in an inability of the adrenals to alter F levels upon subsequent stress. The existence of F-like immunoreactivity and its alteration by environmental manipulation, suggest that it may play a unique physiological role.
OSTI ID:
6299100
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 31:16-17; ISSN LIFSA
Country of Publication:
United States
Language:
English